糖代谢关键酶活性对林可霉生物合成影响的研究

The Influence of Key Enzyme in Glucose Metabolism on Lincomycin Biosynthesis

研究了林可霉素糖代谢的关键酶,己糖激酶、6-磷酸葡萄糖脱氢酶、磷酸果糖激酶和异柠檬酸脱氢酶,以及它们在发酵过程中的活性变化与菌体代谢的变化规律,比较了菌株W11-2和S-41在发酵过程中酶活性的变化趋势,进一步阐明了林可霉素合成和糖代谢关键酶活性的关系。研究表明在发酵的生长阶段,提高糖代谢GK、PFK、G6PDH的活性有利于菌体生长;己糖激酶、6-磷酸葡萄糖脱氢酶的活性和林可霉素合成有正相关性。在林可霉素合成期提高HMP途径的代谢流量,减少EMP途径的代谢流量有利于林可霉素的合成,该研究为发酵工艺的调控和菌种代谢工程的改造提供依据。

The changes of the specific activities of the enzymes involved in glucose metabolism, such as glucokinase, phosphofructokinase, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase were assayed during the synthesis of lincomycin. In addition, the relations between the fermentative process and the mycelium metabolism were also determined. The results in comparing the activities of the enzymes of the strain Wll-2 and strain S-41 showed that bacteria growth was favored by increasing activities of glucokinase, glucose-6-phosphate dehydrogenase, and phosphofructokinase, and the activities of glucokinase and glucose-6-phosphate dehydrogenase have the positive relativity to the biosynthesis of lincomycin. In the product synthesis phase, the metabolism current capacity should enhance the HMP and reduce it in the EMP. This research provides the basis for the fermentation process regulation and metabolism project for strain modification.