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鹿流行性出血病毒分子生物学研究进展 被引量：2

Advance in Molecular Biology of Epizootic hemorrhagic disease virus
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摘要 鹿流行性出血病毒是一种在全世界野生及驯养的有蹄动物中广泛存在的重要病原体。该病毒属呼肠病毒科环状病毒属,有12个血清型,是一种有10个节段(L1-L3、M4-M6、S7-S10)的双链RNA病毒,编码10个蛋白(VP1-7和NS1、NS2、NS3/NS3A)。VP7蛋白具有很强的抗原性且保守性最高。VP2与病毒型特异性有关,可诱导产生中和抗体。VP3为群特异性抗原,高度保守,具有亲水性保守区域。非结构蛋白NS1、NS2和NS3/NS3A及其编码序列均相当保守。该病毒的分子生物学诊断技术主要有PCR和核酸探针杂交技术。 Epizootic hemorrhagic disease virus（EHDV） is a significant pathogen of wild and domestic ungulates worldwide.EHDV is a member of the genus Orbivirus in the family Reoviridae and it has twelve different serotypes.EHDV has ten segmented double-strand RNA genome（L1-L3,M4-M6,S7-S10）, which encode ten proteins（VP1-VP7 and NS1,NS2,NS3/NS3A）.VP7,the most conserved protein of virion,has strong antigenicity.VP2 is a serotype-specific antigen and it can react with neutralized antibody of the virus.VP3,highly conserved protein of the virion,is also a serogroup-specific antigen,and it has the hydrophilic conserved regions.The non-structural proteins NS1,NS2 and NS3/NS3A and their sequences of nucleic acid are highly conserved.The common molecular bio-techniques used for diagnoses of the virus are RT-PCR and nucleic acid probes.
作者 江海天 周晓黎 范晴 孙洪正
机构地区 云南大学生命科学学院 云南出入境检验检疫局技术中心 云南农业大学动物科技学院
出处 《动物医学进展》 CSCD 2007年第12期54-58,共5页 Progress In Veterinary Medicine
基金 国家863项目(2006AA10Z445)
关键词 鹿流行性出血病毒 结构蛋白 非结构蛋白 分子生物学诊断技术 Epizootic hemorrhagic disease virus structural proteins non-structural proteins molecular biological diagnostic techniques
分类号 S852.659.4 [农业科学—基础兽医学]
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参考文献16

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二级参考文献9

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共引文献1

  • 1董仙兰,韩佃刚,董俊,杨云庆,祝贺,叶玲玲,张冲,尹尚莲,李瑶瑶,何昌霖,罗倩敏,艾军.流行性出血病研究进展[J].上海畜牧兽医通讯,2021(4):37-41.

同被引文献26

  • 1陈志飞,李树清.鹿流行性出血热病毒[J].中国进出境动植检,1996(4):31-33. 被引量：6
  • 2刘玉洪,花群义,徐自忠,杨云庆,周晓黎,董俊,尹尚莲,许靖逸,高洪.小反刍兽疫病毒H糖蛋白基因原核表达载体的构建及表达[J].中国兽医科学,2006,36(9):692-695. 被引量：11
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  • 7Meeham J O, Stallkneeht D, Wilson W C. The S7 gene and VP7 protein are highly conserved among temporally and geographically distinct American isolates of epizootic hemorrhagic disease virus[J]. Virus Res,2003, 94(2) : 129-33.
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引证文献2

二级引证文献6

动物医学进展
2007年 第12期

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