一种用于体外培养细胞的静磁场加载装置

A Static Magnetic Field Loading System for In Vitro Cultured Cells

磁性附着体均存在漏磁,应用于患者口腔中时将对邻近组织施加一定强度的静磁场。磁性附着体磁场对机体组织的生物学效应尚鲜见研究报道。本研究研制了一套细胞静磁场加载装置,并模拟磁性附着体静磁场对体外培养人牙周膜细胞加载。该装置能产生恒定的静磁场且磁场强度可调,体积小巧,可对培养皿、培养孔板等多种规格细胞培养器皿中的细胞加载静磁场,便于对细胞的多项生物学指标进行研究。对人牙周膜细胞加载静磁场的结果显示该装置可以有效地对体外培养细胞进行不同强度、不同时间的静磁场加载。该装置为研究静磁场细胞生物学效应提供了必要的手段。

Magnetic attachments have flux leakages, thus they will exert certain magnetic fields on the adjacent tissues when used in the patients＇ oral cavities. There are few research reports on the biological effects of the magnetic fields generated by magnetic attachments on human body. A cellular static magnetic field （SMF） loading system was developed in this study. By using this system, in vitro cultured human periodontal ligament cells （HPDLCs） were loaded with SMF simulating those of magnetic attachments. The cellular SMF loading system could produce constant SMF and the strength of the SMF is adjustable. The system is small and is able to exert SMF to cells cultured in different culture vessels such as culture dishes and culture plates, thus is suitable to researches in multiple biological items of cells. The results of the SMF loading experiment on HPDLCs showed that this cellular SMF loading system could effectively load cells with SMF of different strengths for different time in vitro. The development of this system has provided a useful tool for the researches on the cellular biological effects of SMF.