基质金属蛋白酶在体外视网膜色素上皮细胞损伤模型中的表达

Expression of matrix metalloproteinases in a retinal pigment epithelium cell wound healing model

目的:建立体外RPE细胞损伤模型,观察损伤诱导的视网膜色素上皮(retinal pigment epithelium,RPE)细胞移行以及MMP-2和MMP-9的表达。方法:在近铺满期视网膜色素上皮(retinal pigment epitheli-um,RPE)细胞培养板上,采用角膜移植用的环钻和棉签法做圆形细胞刮伤区,SABC免疫组织化学方法检测RPE细胞受创后48hMMP-2和MMP-9的表达;并应用此损伤模型,计数进入缺损区的细胞数,观察地塞米松(Dex)对RPE细胞移行和对MMP-2及MMP-9表达的影响。结果:RPE细胞受创后损伤边缘的RPE细胞逐渐向缺损区移行;免疫组化结果显示,损伤后48h损伤边缘移行的RPE细胞呈MMP-2的阳性表达,MMP-9呈强阳性表达。Dex处理组中损伤诱导的RPE细胞移行明显减少,MMP-2和MMP-9的表达也明显减弱。结论:MMPs在RPE细胞移行修复中有重要意义,Dex可以通过抑制MMP-2和MMP-9的表达而减少RPE细胞移行。

AIM： The migration of retinal pigment epithelium （RPE） cells is an initial step in the development of proliferative vitreoretinopathy （PVR）. How to reduce the migration of RPE cells is an important focus of research. Matrix metalloproteinases （MMPs） play important roles in migration of cells and remodeling of extracellular matrix. To investigate migration of RPE cells and the expression of MMP-2 and MMP-9 in the RPE cell wound healing model. METHODS： Cultured human RPE confluent monolayers were denuded with a trephine and a cotton stick. Immunohistochemistry were used to detect the expression of MMP-2 and MMP-9 protein in wounded RPE cells at 48 hours after injury. RPE cell migration was measured with the number of RPE cells that entered the denuded area with or without Dexamethasone （Dex）. The influence of Dex on the expression of MMP-2 and MMP-9 was also observed. RESULTS： RPE cells at the edge of scrape migrated into the denuded area after wounded. Immunohistochemistry indicated the positive expression of MMP-2 in RPE cells at the edge of scrape at 48 hours after wounded, and strong positive expression of MMP-9 was observed in motile RPE cells at the edge of scrape. Dex significantly inhibited the migration of wound-induced RPE cells, and the expressions of MMP-2 and MMP-9 were also reduced. CONCLUSION： The migration of RPE cells is an important course in the wound healing, and MMPs play important roles in the migration of RPE cells. Dex could reduce the migration of RPE cells by inhibiting the expression of MMP-2 and MMP-9.