VEGF siRNA对兔眼碱烧伤后角膜新生血管的抑制作用

The inhibitory effect of VEGF siRNA on the development of corneal neovascularization after alkaline burn in rabbits

目的:研究血管内皮生长因子小干扰RNA(VEGF small in-terfering RNA,VEGF siRNA)对兔眼碱烧伤后角膜新生血管的抑制作用。方法:普通家兔25只以碱烧伤法(1mol/LNaOH溶液)诱导角膜新生血管(CNV)生成。碱烧伤后立即以脂质体(LF2000)为载体,右眼球结膜下注射VEGF siRNA重组质粒,左眼球结膜下注射pSilencer 2.1-U6 hygro空白质粒作为阴性对照。碱烧伤后1,3,5,7,14d,形态学分析评价角膜新生血管的生长情况。结果:与对照眼相比,碱烧伤后球结膜下注射VEGF siRNA重组质粒,实验眼在各时间段(3,5,7,14d),CNV长度明显变短,面积明显变小,差异有统计学意义(P<0.05)。结论:VEGF siRNA能有效地抑制碱烧伤后CNV的形成。

AIM： To explore the inhibitory effect of VEGF siRNA on the development of corneal neovascularization（CNV） after alkaline burn in rabbits. METHODS： CNV was inducedin vivo by alkaline burn （1mol/L NaOH） of the central cornea in 25 rabbits. With liposome （ LF2000 ） as vector, VEGF siRNA recombinant plasmid was injected into the subconjunctiva of the right eye , and pSilencer 2. 1-U6 hygro blank plasmid was injected into the subconjunctiva of the left eye as control. At 1,3,5,7,14 days after alkaline burn, CNV was evaluated with morphometric analysis. RESULTS： Compared with the control eyes, the length and the area of CNV in the eyes treated with VEGF siRNA recombinant plasmid after alkaline burn were shortened and reduced significantly（ P 〈 0.05）. CONCLUSION ： VEGF siRNA can effectively suppress the development of CNV after alkaline burn in rabbits.