汉族人群去甲肾上腺素转运体T182C基因多态性与人格特征的关联性

Relationship between T182C gene polymorphism and personality traits in Han population

目的:目前,关于去甲肾上腺素转运体基因T182C多态性与人格关联性的报道较少见,仅有的研究多以健康人群为对象。探讨汉族人群去甲肾上腺素转运体T182C基因多态性与人格特征的关联性。方法:选择2004-04/2005-04于山西医科大学第一医院精神卫生科门诊就诊的汉族重性抑郁患者100例和同期本院汉族健康体检者100名。本次调查于2005-05完成。人格评定采用Eysency编制,龚耀先修订的艾森克人格问卷,计算精神质,内外向,神经质3个个性维度的标准分,并以50分为界,将各维度分为两个等级:非精神病倾向型(精神质<50),精神病倾向型(精神质≥50);内倾型(内外向<50),外倾型(内外向≥50);非神经质倾向型(神经质<50),神经质倾向型(神经质≥50)。取肘静脉血5mL,不加抗凝剂,弃血清,血凝块常规酚氯仿提取基因组DNA,进行PCR分析。PCR产物应用焦磷酸测序PSQ96MA测序仪进行SNP检测,用质谱法进行及等位基因频率分析。检测T,C两种等位基因。结果:纳入的100例重性抑郁患者和100名健康体检者均进入结果分析。男性健康人群检测结果:在精神质维度,C,T等位基因均数分别为46.32±8.17和42.42±7.77,二者比较,差异明显(t=-2.094,P=0.039);C等位基因频率在精神病倾向型人格中为45.5%,明显高于非精神病倾向型人格(22.7%,χ2=4.190,P=0.041);男性重性抑郁患者检测结果:不同等位基因艾森克人格问卷各维度均数及频数分布的比较均无明显差异(P>0.05)。女性检测结果:无论是健康人群还是重性抑郁人群,不同等位基因间艾森克人格问卷各维度均数比较以及艾森克人格问卷各维度分级后的频数分布均无显著性意义(P>0.05)。结论:去甲肾上腺素转运体基因T182C多态性的C等位基因可能是健康男性精神病倾向型人格和重性抑郁男性神经质倾向型人格的危险因素。

AIM： At present, the report about norepinephrine transporter （NET） T182C polymorphism and personality is very few and the former studies are. mostly about healthy population. This article is aimed to explore the relationship between T182C gene polymorphism and personality traits in people of Chinese Han. METHODS： Totally 100 Chinese Han patients with major depression and 100 healthy Han persons who did body examination were enrolled from Out-Patient Clinic of Department of Mental Health, First Hospital, Shanxi Medical University from April 2004 to April 2005. This investigation was done in May 2005. Personality traits were evaluated by Eysency Personality Questionnaire （EPQ） that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism （P）, Extraversion （E） and Neuroticism （N） were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades： non-psychotic type （P 〈 50）, psychotic type （P ≥ 50）, introversion type （E 〈 50）, extraversion type （E ≥ 50）, non-nervous type （N 〈 50） and nervous type （N ≥ 50）. 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry. T and C alleles were determined. RESULTS： A total of 100 patients with major depression and 100 healthy persons who did body examination were involved in the result analysis. Detection result of male healthy population showed that means of C and T alleles in P dimension were 46.32±8,17 and 42.42±7.77, respectively, and there were significant differences （t =-2,094, P =0.039）. C allele frequency （45.5%） in psychotic type was obviously higher than that in the non-psycho＇tic type （22.7%,Х^2=4.190, P =0.041 ）. Detection result of male population with severe depression： There was no significant difference in mean and frequency distribution of allele of different level in EPQ. Detection result of females showed that no matter healthy population or population of severe depression, there was no significant difference in the mean of different allele and the frequency after grading in each dimension of EPQ（P〉 0.05）, CONCLUSION： C allele of T182C polymorphism may be the risk factor of healthy male with psychotic type and major depression male with nervous type.