海洋氧化短杆菌15E产碱性蛋白酶的发酵条件

Fermentation conditions of alkaline protease from marine bacterium strain 15E(Brevibacterium oxydans)

对氧化短杆菌15E菌株(Brevibacterium oxydans)产碱性蛋白酶的发酵条件进行研究,结果表明,培养基初始pH值9.0、培养温度28℃、摇床转速200.rmin-1条件下培养40 h,菌体生长最适且总酶活力达到最大;菌株在添加5 g.L-1牛肉膏、1 g.L-1葡萄糖的酪蛋白培养基中生长和产酶均达到最佳;人工海水中的K+、Ca2+和Mg2+浓度较为适合15E菌株生长和产酶,其中K+对菌株产酶起关键作用.

Fermentation conditions of alkaline protease of strain 15E （ Brevibacterium oxydans） were researched. The results showed that the maximum enzyme activity and biomass could be achieved when strain 15E was cultured at 28 ℃ for 40 hours in casein medium （pH 9.0） with shaking speed200 r . min^-1. Casein medium with 5 g · L^-1 beef extract and 1 g . L^-1 glucose added was suitable for the growth of the bacteria and their production of protease. The concentration of K^＋ , Ca^2＋ and Mg^2＋ in artificial seawater was suitable for growth and protease synthesis of strain 15E, while K ^＋ was important for protease production.