骨髓间充质干细胞对大鼠肺组织胶原的影响

Effect of bone marrow-derived mesenchymal stem cells on collagen of injured rat lung

目的:观察骨髓间充质干细胞(MSCs)对博来霉素(BLM)引起的肺损伤大鼠肺组织胶原的影响,初步探讨其作用机制。方法:将SD大鼠MSCs异体注入受体大鼠体内,2周后观察肺组织病理学变化,同时检测肺组织羟脯氨酸含量及转化生长因子β1(TGFβ1)、血小板源性生长因子-A、B(PDGF-A,B)、胰岛素样生长因子-Ⅰ(IGF-Ⅰ)mRNA的变化。结果:MSCs的植入可使增生的大鼠肺泡间隔、基质胶原及Ⅰ、Ⅲ型胶原明显降低,使升高的肺组织羟脯氨酸及TGFβ1、PDGF-A,B、及IGF-ⅠmRNA的表达显著降低。结论:MSCs可减少肺损伤大鼠肺组织中的胶原含量,此作用与MSCs降低了多种促纤维化细胞因子的表达有关。

Objective ：To investigate the effect of bone marrow -derived mesenchymal stem cells （MSCs） on collagen of injured rat lung induced by bleomycin and its mechanism. Methods： MSCs, isolated from SD rats, were injected into recipient rats. Two weeks later, lung tissues were collected and lung structure, collagen Ⅰ ,Ⅲ and hydroxyproline were detected. The expression of TGFβ1, PDGF - A, PDGF - B and IGF - Ⅰ mRNA were also examined. Results： The thickness of alveolar wall, collagen Ⅰ ,Ⅲ and hydroxyproline in lung tissue in MSCs treatment group were reduced significantly than those in lung injury group. MSCs engraftment can also reduce the expression of TGFβ1, PDGF - A, PDGF - B and IGF - Ⅰ mRNA in injured lung. Conclusion ： MSCs could reduce the collagen in injured lung and this effect may be associated with the decrease of TGF - β1, PDGF - A, PDGF - B and IGF- Ⅰ expressions induced by MSCs engraftment.