白藜芦醇对肿瘤细胞HL-60代谢热化学研究

Study on the Thermochemical Characteristics of the Inhibitory Action of Resveratrol on the HL-60 of Cancer Cell

目的:用微量热法研究了白藜芦醇对白血病细胞HL-60代谢作用的热化学特征。方法:等温量热仪测定HL-60细胞在白藜芦醇作用下生长代谢的热功率输出曲线。结果:白藜芦醇在20μg/mL～80μg/mL范围内,热曲线一直处于缓慢下降状态,对该肿瘤细胞有明显抑制作用,当计量达到100μg/mL～120μg/mL时,热功率曲线值呈缓慢增加趋势,抑制效果变弱。结论:白藜芦醇对细胞HL-60代谢有明显的抑制作用,为白藜芦醇在防治肿瘤中的应用提供了可靠的信息。

Objective： The micro calorimetric method was applied to the study on the thermochemical characteristics of the inhibitory action of resveratrol on the metabolism of leukaemia cell HL- 60. Methods： Micro- calorimetry mensurate the export curve of the thermochemical on the cell HL- 60 adding resveratrol. Results： The thermochemical curve slowly decline,and have remarkable inhibitory effect for the cell when the resveratrol＇s concentration is from 20μg/mL to 80μg/mL. But when the resveratrol＇ s concentration is from 100μg/mL to 120μg/mL, the curve shows increase tardiness, and the inhibitory effect become weak. Conclusion： It was shown by the experimental results that resveratrol had remarkable inhibitory effect on the metabolism of the leukaemia cell HL- 60, and that supply reliable information for prevention and cure of tumour （benign or malignant）.