中华蟾蜍与黑斑蛙消化道黏液细胞的观察

Study on Distribution and Type of Mucous Cells in Digestive Tract of Bufo bufo gargarizans and Rana nigromaculata

目的:探索两栖类动物消化道黏液细胞的类型与分布规律。方法:利用阿利新蓝与过碘酸雪夫试剂染色法对中华蟾蜍(Bufo bufo gargarizans)、黑斑蛙(Rana nigromaculata)消化道黏液细胞进行石蜡切片和染色。结果与结论:黏液细胞表现为4个类型:Ⅰ型玫瑰红色;Ⅱ型蓝绿色;Ⅲ型紫红色;Ⅳ型蓝紫色。两种动物食管黏膜上皮中黏液细胞主要是杯状细胞,其中中华蟾蜍的黏液细胞主要为Ⅱ型和Ⅳ型细胞,黑斑蛙的黏液细胞主要Ⅲ型细胞。胃体柱状黏膜上皮细胞与胃腺颈细胞主要为Ⅰ型和Ⅲ型细胞,黏原颗粒主要集中在细胞的核上区。胃腺浅部有成团分布并着色较浅的黏液细胞,其中黑斑蛙胃腺黏液细胞主要为Ⅰ型和Ⅲ型细胞,中华蟾蜍胃腺黏液细胞主要为Ⅲ型和Ⅳ型细胞。小肠杯状细胞主要为Ⅲ型和Ⅳ型细胞。大肠杯状细胞主要为Ⅱ型和Ⅳ型细胞。

Objective： To study distribution and morphology of mucous ceils in digestive tract of amphibian. Methods： Mucous ceils in digestive tract of Bufo bufo gargarizans and Rana nigromaculata are studied by using the AB（alcian blue） staining, PAS（periodic acid schiff＇ s reaction） staining, AB - PAS combined staining. Results and conclusion： The mucous ceils appear four types after they were steined with AB - PAS com- bined staining mothed： Type Ⅰ , in rose- red colour; Type Ⅱ, in green- blue colour; Type Ⅲ, in purplish- red colour; Type Ⅳ, in purplish - blue colour. The mucous cells in oesophagus of the animals are goblet ceils, among them, the mucous cells of Bufo bufo gargarizans mainly belong to Type Ⅱ and Ⅳ, the mucous cells of Rana nigromaculata mainly belong to Type Ⅲ. The mucosal epithelium and mucous neck ceils of fimdic gland in stomach mainly belong to the Type Ⅲ and Type Ⅰ , of which the mucous granules are being concentrated in their shallow part of the ceils; and the mucous ceils of fundic gland, cardiac gland, pyloric gland of Rana nigromacuiata mainly belong to Type Ⅲ and Type Ⅰ , but the mucous cells of Bufo bufo gargarizans mainly belong to Type Ⅲ and Ⅳ, with slightly staining. The goblet cells in all part intestinum tenue of the animals mainly belong to Type Ⅲ and Ⅳ. The goblet ceils in all part intestinum crassum of the animals mainly belong to Type Ⅱ and Type Ⅳ.