胡杨器官和体胚发生方式的植株再生

Regeneration of Populus euphratica via Organogenesis and Embryogenesis

目的:为以胡杨为亲本的体细胞杂交育种奠定基础。方法:以胡杨苗叶片为外植体,通过器官和体胚两种不同发生方式建立了离体再生体系。结果:附加0.75mg/LBA、0.5mg/LNAA基本培养基及3w暗培养是愈伤组织诱导的最佳条件;附加0.25mg/LBA和0.1mg/LNAA的基本培养基上不定芽的诱导率最高;1/2大量元素的MS培养基附加0.1mg/lNAA、0.05mg/L和1.5%蔗糖对不定芽生根效果最好;诱导并筛选出的胚性愈伤组织在附加了0.5mg/LBA、0.5mg/LNAA的基本培养基上诱导获得大量胚状体,干化处理后大部分能经子叶胚期萌发成苗。结论:外植体的采集周期和培养条件影响胡杨离体叶片的形态发生途径。

Objective： The research laid a foundation for somatic hybridization with Populus euphratica as parental source. Methods： Using leaves of seedling as explant, we obtained regenerated plantlets of Populus euphratica via organogenesis and embryogenesis. Results： The callus induced from leaf explant were optimized in the basal medium containing 0.75mg/L 6 - BA and 0.5mg/L NAA with 3 - week darkness culture; the basal medium with 0.5 mg/L BA and 0.1 mg/L NAA promoted shoots differentiation; shoots were bested rooted on half- strength micro - salt MS medium containing 0.05 mg/L IBA and 0. 1mg/L NAA; the embryogenie callus was optimized on the basal medium fortified with 0.5mg/L BA and 0. 5mg/L NAA, on which developed the most abundant embryos. After the process of maturation, most embryos developed to the eotyledonary stay and germinated into shoots. Conclusion： The collection period and culture condition affect the morphogenesis via organogenesis or embryogenesis.