矿物培养影响微生物群落的ERIC研究

Study on the Changes of Microbial Ecology with ERIC Method after Cultivation by Different Mineral Resources

从江西东乡铜矿、江西银山铅锌矿和广东云浮硫铁矿三地采集了酸性矿坑水样品,用ERIC方法(肠细菌重复内源同源序列方法)分析了经不同矿物培养后的微生物变化情况。结果发现:不同矿山酸性矿坑水样品的化学性质及其微生物组成存在很大差异,同一样品经不同矿粉培养后微生物群落也会发生比较大的变化;样品经组成相似的能源物质培养后,其微生物群落结构也越相似。确定了ERIC方法用于酸性矿坑水样品时的最佳扩增体系和反应程序。

Three samples of acid mine drainage were collected from the Dongxiang copper mine in Jiangxi province, the Yinshan lead-zinc mine in Jiangxi province and the Yunfu sulfide mine in Guangdong province. After the samples were cultivated by different energy resources, the microbial communities were analyzed by enterobacterial repetitive intergenic consensus-PCR （ ERIC-PCR ） method. The results show that the chemical characters and microbial communities of the samples are very different. The microbial communities would be much different even the same sample was cultivated by different minerals as energy resources. It is also shown that similar compositions of the energies for cultivation result in more similar microbial communities. Furthermore, the reaction system and program of ERIC-PCR method for the acid mineral drainage were optimized.