三七茎叶总皂苷酶转化产物和C-K的抗肿瘤作用

Anti-tumor Effects of Ginsenoside Compound K and the Enzymatic Transformed Products from the Total Saponins in Leaves of Panax notoginseng by β-Gglucanase

目的:研究三七茎叶总皂苷酶转化产物和人参皂苷化合物K(C-K)的抗肿瘤作用。方法:利用β-葡聚糖苷酶转化三七茎叶总皂苷,转化产物经大孔吸附树脂富集和纯化,硅胶柱层析及制备薄层层析分离纯化后得到C-K。观察酶转化产物和C-K对S180肿瘤细胞生长的抑制作用及对艾氏腹水癌小鼠生存期的影响。结果:三七茎叶总皂苷酶转化产物和C-K的抑瘤率分别为41.8%和44.7%;对艾氏腹水癌小鼠延长存活期分别为3.9d和4.6d。结论:三七茎叶总皂苷酶转化产物和C-K具有较强的抗肿瘤作用。

Objective ： To study the anti - tumor effects of ginsenoside compound K （ C - K） and the enzymatic transformation products （ETP） from the total saponins in leaves of Panax notoginseng （SLPN） by β- glucanase. Methods： An industrial enzyme β -glucanase was used to transform SLPN. ETP were extracted by D - 101 macroreticular resin, separated and purified by column chromatographies on silica gel and thin layer gel. One of the main compounds was identified as： ginsenoside C -K [20 （S） -protopanaxadiol -20 -O -β- D -glucopyranoside, C - K] , the structure of C - K was elucidated on the basis of NMR spectral data. The results of inhibition growth on rats loaded with S180 sarcomata cell and the influence of the duration for Ascites cancer of rats by ETP and C -K were observed. Results： The antitumor experiment showed that the inhibition tumor rate （ITR） of ETP and C - K were 41.8% and 44.7% , and the duration for Ascites cancer of rats were prolonged 3.9days and 4.6days by ETP and C - K separately. Conclusion ： The antitumor effects of ETP and C - K were very obviously.