丹参酚酸B的微生物转化

Microbiological Transformation of Salvianolic Acid B

目的:通过真菌对丹参酚酸B进行转化,获得新的酚酸类活性物质,建立新的获得药源的方法。方法:采用微生物发酵技术培养真菌,进行转化;采用RP-HPLC方法,检测及制备转化产物;利用理化性质和波谱学分析,鉴定转化产物的化学结构。结果:观察了150种真菌,发现一株真菌可高产率转化丹参酚酸B(1),转化后得到的产物,经鉴定确定为原紫草酸(2)。结论:该产物用化学合成的方式很难得到。

Objective： To get new bioactive salvianolic acid products and set up new method to obtain medicinal resource. Methods： The salvianolic acid B was transformation by fermentation technique; RP - HPLC was used to detect and prepare the product; the structure of product was identified by the spectral analysis and chemical evidence. Results： One salvianolic acid was isolated and identified as prolithospermic acid （1） after screening 150 strains of fungi. Conclusion： Prolithospermic acid （1） can not got easily by chemical methods.