摘要
目的观察一种防治瘢痕的外用复方中药瘢痕膏对兔耳增生性瘢痕挛缩的抑制作用。方法选用8只新西兰大耳白兔建立兔耳增生性瘢痕动物模型。实验组于创面上皮化时即开始用瘢痕膏,并设立空白对照。用药后第30天及第100天观察两组瘢痕组织的外观形态,应用ABC免疫组织化学法染色测α-平滑肌肌动蛋白(α-SMA),利用计算机辅助病理图象分析系统测定并纪录每张切片阳性反应区域的平均面积百分比和光密度值。结果实验组瘢痕反应较轻,免疫组化染色瘢痕膏组兔耳瘢痕组织α-SMA平均面积百分比和光密度值明显低于对照组(P<0.05)。结论用复方中药对兔耳增生性瘢痕挛缩有抑制作用,对增生性瘢痕有防治作用。
Objective To observe the effect of a compound traditional Chinese medicine ointment on the prevention of rabbit ears hypertrophic scar. Methods Eight New Zealand rabbits were selected as experiment animals. The ointment began to be used in the experimental group when the wound healed completely while nothing was done to the controls. Respectively , after 30 days and 100 days the appearance of scar tissue morphology were observed, and the α-smooth muscle actin （α-SMA） was tested by immunohistochemical staining. Computer - aided image analysis system was used to determine and record the percentage of positive region and the optical density in each slice. Results The percentage of α-SMA positive region and the optical density in the experimental group are both significantly lower than that in the control group （ P 〈 0.05 ）. Conclusion The ointment can inhibit the hypertrophic scar contracture and prevent hypertrophic scars of rabbit ears.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第12期2941-2943,共3页
Lishizhen Medicine and Materia Medica Research
基金
广西医疗卫生重点科研课题基金(No.200314)
