小鼠血浆葛根素的含量及其药代动力学研究

Determination of Peurarin and Pharmacokinetic in Mouse Plasma

目的小鼠血浆中葛根素的含量及药代动力学研究。方法采用高效液相色谱法,流动相为甲醇-水(23∶77),流速1.0 ml.min-1,检测波长250 nm,柱温为25℃。结果葛根提取物口服后15min血液中即可检出葛根素,在体内呈二室模型分布,其主要药动学参数为A=1121.105 mg.L-1;B=237.5469 mg.L-1;α=0.6825 h-1;β=0.0459 h-1;Kα=1.3693 h-1;t1/2α=1.015 6 h;t1/2β=15.0984 h;K10=0.13 h-1;K21=0.230 5 h-1;K12=0.362 h-1;Cmax=456.637 6 mg.L-1;Tmax=1.242 7 h;AUC=5 824.714 4 mg.h.ml-1。结论葛根提取物口服后葛根素在小鼠体内吸收快、消除慢。

Objective To determine the peurarin and pharmacokinetic in mouse plasma. Methods HPLC method was used in the study . The analysis was carried out on Phenomenex Luna C18 column. The mobile phase was MeOH -H2O （23： 77）. Flow rate 1.0 ml·min^-1, wavelength 254 nm, temperature 25℃. Results The results indicated that peurarin was detected after p.o. 15 min and the plasma C - T curve conform to two - compartment open model. The Pharmacokinetic parameter was below ： A = 1 121,105 mg·L^-1;B=237.5469 mg·L^-1;α=0.6825 h^-1; β=0.0459 h^-1;Kα=1.3693h^-1 ;t1/2 = 1.0156h;t1/2β= 15.098 4 h;K10 =0.13 h^-1;K21 = 0.2305 h^-1;K12=0.362 h^-1; Cmax =456.637 6 mg·L^-1;Tmax = 1.2427 h;AUC =5 824.7144 mg·h·ml^-1. Conclusion The peurarin is absorbed very fast and eliminates slow in mice.