Troglitazone对小鼠Ⅱ型葡萄糖载体和Ⅰ型葡萄糖载体mRNA表达的影响

Effect of Troglitazone on Mouse Glucose Transporter Ⅱ (mGLUT2) and Mouse Glucose Transporter I (mGLUT1) mRNA Expression

目的探讨Troglitazone对小鼠Ⅱ型葡萄糖载体(mGLUT2)和I型葡萄糖载体(mGLUT1)mRNA表达的影响。方法采用分子克隆技术,将过氧化物酶体增殖物受体γ(PPARγ)和维甲酸类受体X(RXRα)及mGLUT2和mGLUT1cDNA分别克隆到表达载体pCMX和pGL3b上。PPARγ与RXRα及mGLUT2与mGLUT1克隆载体转染NIH3T3细胞,处理或不处理Troglitazone,应用荧光素酶活性测定法及RNA印迹等方法测定Troglitazone对mGLUT2和mGLUT1重组体荧光素酶活性调节及对mRNA表达的影响。结果Troglitazone可激活mGLUT2和mGLUT1重组体荧光素酶的活性,并且可增加mR-NA的表达水平。结论Troglitazone可增强mGLUT2和mGLUT1的表达,可能参与PPARγ对mGLUT2和mGLUT1的调节过程。

Objective To understand the possibility of the effect of Troglitazone on mGLUT2 and mGLUT1 mRNA expression. Methods PPARγ, , RXRα and mGLUT2 and mGLUT1 cDNA were cloned into pCMX and pGL3b expression vector. The recombinant DNA transfected into NIH 3T3 cells, treated Trogihazone or not. The mGLUT2 and mGLUT1 activity and mRNA expression were determined by the Trogiltazone through PPARγ activation with luciferase assay and Northern blot. Results Troglitazone activated the mGLUT2 and mGLUT1 activity and increased the mGLUT2 and mGLUT1 mRNA expression through PPARγ activation. Conclusion Troglitazone perhaps binds to the regulations on mGLUT2 and mGLUT1 by PPARγ.