土鳖虫含药血清与化疗药物联合对人肝癌细胞增殖的影响

Synergism suppression of eupolyphaga cream contained serum and chemotherapeutic agents on human hepatoma HepG-2 cell line

目的:观察土鳖虫含药血清作用前后肝癌HepG-2细胞化疗药物相关基因表达水平的改变,探讨其可能的分子机制。方法:以肝癌HepG-2细胞为研究对象,使用实时荧光定量RT-PCR方法检测土鳖虫含药血清作用前后肝癌HepG-2细胞化疗药物相关基因TS、ERCC1表达水平的变化。采用MTT法检测10%土鳖虫含药血清与5-FU、DDP单独、联合用药对肝癌HepG-2细胞增殖的抑制作用。结果:实时荧光定量RT-PCR结果显示,10%土鳖虫含药血清对TS基因表达有下调作用,对ERCC1基因表达有上调作用。药物敏感实验中,在单用土鳖虫含药血清对肝癌HepG-2细胞还未产生抑制作用时,联合5-FU或DDP用药抑制率即高达69.315%或74.775%,显示土鳖虫含药血清与5-FU或DDP联合用药有明显的协同作用。结论:土鳖虫乳剂作为一种新型抗肿瘤中药制剂,经过动物体内代谢后所得血清与常用化疗药物5-FU、DDP有明显的协同作用,可能与药物相关重要基因表达的变化有关,但与DDP协同作用的机理有待进一步研究。

Objective： To inestigate the influence of the eupolyphaga cream contained serum on chemotherapeutic agents associated genes in vitro. Methods： The human hepatoma HepG-2 cell lines was used in the study. The influence of the serum on chemotherapeutic agents associated genes （TS and ERCC1 ） was measured with real time quantitative PCR （RT-PCR）. MTT assay was used to authenticate the result of the gene test. Results： The anticancer drug associated gene such as TS was down regulated, while ERCC1 was up regulated. The treatment of HepG-2 cell with the serum combination with 5-FU or DDP could significantly increase the antineoplastic activity in vitro. Conclusion： Eupolyphaga cream as a new poweful antineoplastic herb praeparatum promotes the development of traditional Chinese medicine anticancer ressarch.