原位杂交法检测正畸力作用下大鼠牙周组织表皮生长因子-mRNA的表达

Expression of epidermal growth factor receptor-mRNA in rat periodontal tissues during orthodontic tooth movement

目的检测正畸力作用下大鼠牙周组织中表皮生长因子受体-mRNA(EGFR-mRNA)的表达与分布,探讨EGF在正畸牙移动过程中的作用及机制。方法建立大鼠正畸牙移动模型,分别在受力24h及168h处死,制备左侧上颌第一磨牙及牙周组织的石蜡标本,采用地高辛标记的原位杂交法检测样本牙周组织EGFR-mRNA的表达与分布。结果EGFR-mRNA表达的强度高于受力24h组(P<0.01);同时间组中张力侧EGFR-mRNA的表达高于压力侧(P<0.01);EGFR-mRNA在正畸组中的表达主要是位于近远中向的张、压力侧,而生理状态下主要位于根尖及根分叉区的牙周膜。结论正畸牙移动过程中EGFR-mRNA的表达增强,提示EGF可能从基因水平参与了正畸牙移动,促进了组织的修复形成。

[Objective] To detect the expression and distribution of epidermal growth factor receptor-mRNA （EGFR-mRNA） in rat periodontal tissues, and to analyze its role in the remodeling of tissue during orthodontic tooth movement. [Methods] According to Kings methods, 40 g mesial force was applied to pull the left maxillary first molar in the rat. Using in situ hybridization technique with a digoxigenin labled probe to detect .the expression of EGFR-mRNA in peridontal tissues in rat decalcified alveodental connective tissues at 24 hours and 168 hours of tooth movement. [Results] EGFR-mRNA was stained weakly at some of periodontal ligament of furcation and radical regions in control group. The expressions EGFR-mRNA increased in orthodontic rat periodontal tissues（P〈0.01）, with the expression at 168 hours higher than that at 24 hours （P〈0.01）. And levels of EGFR-mRNA at tension side were higher than those at pressure side at the same time （P 〈0.01）. [Conclusion] Epidermal growth factor receptor participated in the tissues remodeling during orthodontic tooth movement in the level EGFR-mRNA. The results also showed that EGFR-mRNA played a more important role in orthodontic bone in the formation.