红球藻虾青素含量测定方法的探讨

Comparative Analysis of Quantitation of Astaxanthin in Haematococcus pluvialis

研究比较了4种测定雨生红球藻内虾青素含量的方法,包括Cyanotech公司的测定方法,荆州天然虾青素有限公司的方法,氯仿-乙醇混合溶液提取测定方法与高效液相色谱测定法.结果表明:HPLC法测定虾青素的标准曲线R2为0.9996,保留时间的RSD%为1.580%,峰面积的RSD%为2.750%,检出限为0.045μg.mLR2-1,实测的样品虾青素含量为1.638%.Cyanotech公司的测定结果与HPLC法最接近,达到1.461%,在3种分光光度法中测定效果最好,其他分别只达到了1.250%、1.317%。

The four methods of quantization of astaxanthin content in Haematococcus pluvials are investigated and compared. The four methods are that proposed by Cyanotech company, the one used by Jingzhou natural Astaxanthin Inc, the one of mixture solution of chloroform and ethanol, and the one from high performance liquid chromatography （HPLC）. The results show that the R2 of graph standard of astaxanthin content is 0.9996, the RSD% of Rt is 1.58%, the RSD% of peak area is 2.75%, the detective limit is 0.045 μg·mL^-1, and the sample＇s astaxanthin content is found to be 1.638%, as detected by HPLC. The result of 1.4611% is found to be the closest to that reported by the method of Cyanotech company, and the other two reach only 1.25% and 1.317 %. In conclusion, the method of Cyanotech company is best among three methods using spectrophotometer.