经腹主动脉阻断致犬脊髓缺血再灌注损伤实验模型的探讨

Model formation of ischemia-reperfusion injury in spinal cord of canis by occlusion of abdominal aorta

目的 经腹主动脉阻断建立犬脊髓缺血再灌注损伤模型。方法 健康成年家犬14只，其中肾动脉后阻断型8只，阻断时间分别为30、60、90及120min（各2只），肾动脉前阻断型6只，阻断时间分别为60和120min（各3只）。于动脉阻断前、阻断后0、20、40、60、80、100min及动脉开放后0、10、30min测定心率（HR）、桡动脉压（RABP）、股动脉压（FABP）、鼻咽温度（NPT）和直肠温度（RT），再灌注后12、24、48h及7d动物行为学评分。结果 动脉阻断前后HR基本平稳；RABP略有升高，动脉开放后则逐渐趋于平稳，FABP在动脉阻断后波形消失，收缩压及舒张压基本相等，维持在20～40mmHg之间；NPT基本平稳，RT随着阻断时间的延长逐渐降低；再灌注后12h行为学评分均为≥3分，24h以后均为4分。结论 本研究采用的方法确能保证腹主动脉完全阻断，但建立脊髓缺血再灌注损伤模型失败。

Objective To establish a model of ischemia-reperfusion injury in the spinal cord of canis by occluding the abdomical aorta. Methods Fourteen healthy adult canis were divided into two groups ： Group A（ n = 8 ）, in which the abdominal aorta was occluded posterior to the renal artery by an artery forcep for 30,60,90, or 120 min;and Gruop B （ n = 6 ）in which the abdominal aorta was occluded anterior to the renal artery by an artery forcep for 60 or 120 min. Hearth rate （HR ）, radial arterial blood pressure （RABP）, femoral arterial blood pressure （ FABP）, nasopharyngeal temperature（ NPT）, and rectal temperature（ RT）were measured during the procedure. The modified Tarlov criteria scores（MTCS） were evalated after the procedure. Results HR and RABP remained unchangeable during the whole proeedttre. The wave of FABP disappeared when the aorta was occluded. NPT was invariant, while RT decreased as the occlusion time went on. MTCS was more than or equal to 3 at 12h after the reperfusion,and it was equal to 4 at 24h after the reperfusion in all canis. Conclusion Although the abdominal aorta can be occluded completely by an artery forcep, we failed to establish the model of ischemia- reperfusion injury in spinal cord of canis.