双低油菜农杆菌介导法导入草酸氧化酶基因研究

Transfer of oxalate oxidase gene into double-low Brassica napus by agrobacterium-mediated transformation

草酸氧化酶基因(OxO)可将草酸转化为CO2和H2O2,为研究草酸氧化酶基因对植物抗病的作用,本文通过根癌农杆菌(Agrobacterium tumefaciens)介导途径,将草酸氧化酶基因导入了双低油菜,经过诱导分化获得抗卡那霉素抗性的再生植株。经PCR检测得到了与阳性对照一致的470 bp的片段,初步表明OxO基因已整合进油菜基因组中。在诱导接种实验和对草酸的耐受性实验中,转基因油菜植株的抗性明显高于未转化油菜。

OxO was introduced into double-low Brassica napus by agrobaterium-mediated transformation. PCR identification with the kanamycin resistant plants showed the most of the greenplants amplified gave the expected fragment of 470 bp as the positive control. Transgenic plants showed a stronger resistance against oxalic acid and better tolerance against the pathogen than wild type.