连接酶链反应检测沙眼衣原体和淋病奈瑟菌的可重复性问题研究

Study on Reproducibility with the Abbott Laboratories LCx Assay for Chlamydia Trachomatis and Neisseria gonorrhoeae

〔目的〕应用连接酶链反应技术(LCR)检测男性尿标本中的淋病奈瑟菌和沙眼衣原体,发现并阻止污染和可重复性问题的发生。〔方法〕采集受检者晨起或较长时间(2h以上)不排尿后的首段尿(FVU)标本1130份,利用LCR对此尿液标本进行淋病奈瑟菌和沙眼衣原体检测,针对S/CO在0.8以上的标本72h内进行复检,如果重复实验的S/CO≥1.0则认为阳性,S/CO<1.0,则为阴性。〔结果〕在1130例标本中,淋病奈瑟菌的S/CO在0.8以上的标本有13份,重复实验10份为阳性,其中3份S/CO在0.8～2.0的标本,复检为阴性。沙眼衣原体阳性60份,重复实验45份为阳性,18份S/CO在0.8～2.0的标本,15份复检为阴性。〔结论〕采用LCR检测淋病奈瑟菌和沙眼衣原体时,如S/CO在0.8～2.0应进行重新检测,可阻止污染和可重复性问题的发生。

Objective objective To detect and prevent contamination and reproducibility problems of LCR by using Abbott Laboratories LCx Assay for Chlamydia trachomatis and Neisseria gonorrhoeae. Method Neisseria gonorrhoeae （NG） and Chlamydia trachomatis（CT） were examined with LCR plasmid assay in First-void urine（FVU）of 1 130 samples, the results whose sample/cutoff IS/ CO] were above 0,8 should be repeated within 72 hours. If the repeat test S/CO ratio was ≥1 1.0, the result was considered positive, and if it was 〈1.0, it is reported as negative, Result 1 130 samples were performed, of which, 13 specimens whose S/CO were above 0.8 were tested again for NG, 10 samples were positive and 3 samples whose S/CO were in 0.8-2.0 were negative, For CT, 60 specimens whose S/CO were above 0.8 were tested again, 45 samples were positive, of 18 samples whose S/CO were in 0.8-2.0, 15 samples were negative. Conclusion This study demonstrates that the results whose S/CO were in 0,8-2.0 should be repeated during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These procedures can detect and prevent contamination and reproducibility problems,