胎盘感染小鼠巨细胞病毒后的病理学改变

Pathological Changes of Placenta after Intrauterine Murine Cytomegalovirus Infection

目的:观察小鼠巨细胞病毒(MCMV)胎盘感染后胎盘病理学改变。方法:酶联免疫法(ELISA)筛选无MCMV感染史性成熟期雌性小鼠,雌雄同笼受孕后随机分为模型组和对照组,模型组孕龄12.5 d时经胎盘显微注射MCMV悬液,18.5 d时处死,观察胎盘组织标本病理改变;对照组胎盘注射细胞维持液。结果:模型组较对照组胎盘感染率高(P<0.05)。其胎盘细胞滋养细胞增生、合体细胞结节增生、纤维素样坏死等病理学变化,与对照组差异均有显著意义(P<0.05)。结论:胎盘感染MCMV后出现的病理改变可能在MCMV宫内感染进展中发挥重要作用。

Objective To investigate the pathological changes of placenta after intrauterine murine cytomegalovirus （MCMV） infection. Methods Sixteen mature female mice without MCMV infection identified by Enzyme - linked immunoabsorbent assay （ELISA） were served as the subjects. The female mice were randomly divided into model group and control group, and then was injected MCMV into the placentas of the model group mice at day 12.5 of gestation, the control group were injected with cell maintenance medium alone, the female mice was sacrified at day 18.5 of gestation and the pathological changes of their placentas were observed. Results Compared with the control group, the infection rate of placentas was significant higher in model group（ P 〈 0.05 ）, and the pathological changes of placentas, including cytotrophoblast proliferation,syncytiotrophoblast knotting and fibrinoid necrosis were also significantly increased （P 〈 0.05 ）. Conclusions The pathological changes of placentas infected with MCMV could play an important role in development of intrauterine murine cytomegalovirus infection.