去甲斑蝥素和阿霉素对人胆管癌细胞QBC939的联合作用

Effect of norcantharidin combined with adriamycin on human cholangiocarcinoma cell line QBC939

目的研究去甲斑蝥素与阿霉素对人胆管癌细胞QBC939体外作用。方法以体外培养的人胆管癌细胞QBC939为实验模型,分别或联合应用不同浓度的去甲斑蝥素与阿霉素作用于癌细胞,光学显微镜下观察药物处理后癌细胞的细胞形态学变化,四甲基偶氮唑蓝(MTT)比色法分析药物对癌细胞生长的抑制作用,流式细胞技术测定药物作用后癌细胞的细胞周期分布和凋亡率。结果去甲斑蝥素和阿霉素单独作用于癌细胞时都有增殖抑制作用,且呈剂量依赖性(P<0.05)。两药合用对癌细胞的细胞毒作用优于两者的单纯相加。去甲斑蝥素和阿霉素两药联合使用可以使癌细胞阻滞于G0/G1期(P<0.05),凋亡率明显升高(P<0.05)。结论去甲斑蝥素、阿霉素无论单用或合用对癌细胞的增殖均有抑制作用,均能够明显改变癌细胞的细胞周期分布并诱导癌细胞出现凋亡,但二者合用有协同作用。

Objective To explore the effect of norcantharidin and adriamycin on human eholangiocarcinoma cell line QBC939 in vitro. Methods The morphology of the cells was observed by light microscope. The effects of norcantharidin and adriamycin alone or in combination on the growth of htaman cholangiocarcinoma cell line QB939 were detected by MTT assay,and cell cycle and DNA content were detected by flow cytometry. Results Norcantharidin or adriamycin alone could inhibit the growth of QBC939 cells in a dose dependent fashion （P 〈 0.05）, and the combination of norcantharidin with adriamycin showed better inhibitive effect on QBC939 ceils. Using the combination of norcantharidin with adriamycin, cell cycle of QBC939 cells was significantly arrested in C0/G1 phase （P〈0.05）, and the apoptosis rate of QBC939 cells increased significantly（P〈0.05）. Conclusion Norcantharidin and adriamycin in combination has synergistic effect on cytotoxicity, and it could effectively inhibit the growth of QBC939 cells, change the distribution of cell cycle and induce apoptosis of QBC939 cells.