卡巴胆碱对烧伤大鼠肠系膜微循环的影响

Effects of Carbamylchocline on Mesenteric Microcirculation in Burned Rats

目的探讨卡巴胆碱对烧伤大鼠肠系膜微循环的影响。方法采用大鼠烧伤模型,分为对照组、烧伤组和卡巴胆碱治疗组。用活体微循环观测技术观察卡巴胆碱对烧伤大鼠肠系膜微血管管径、血流速度的作用。用激光多普勒血流量仪测肠壁血流量的变化。结果(1)烧伤组大鼠颈动脉平均压和细静脉管径较对照组分别下降19.30%(P<0.01)和9.97%(P<0.05),给予卡巴胆碱治疗后,颈动脉平均压和细静脉管径较烧伤组分别升高17.88%(P<0.05)和29.42%(P<0.001)。(2)烧伤组大鼠肠系膜细静脉血流速度和肠壁血液灌注量较对照组分别下降68.18%和70.66%(P<0.001),卡巴胆碱可明显增加肠系膜细静脉血流速度和肠壁血液灌注量,分别是烧伤组的2.41倍和2.45倍(P<0.001)。(3)烧伤后大鼠毛细血管网开放数较对照组下降55.21%(P<0.001),给予卡巴胆碱治疗后,毛细血管网开放数是烧伤组的1.95倍(P<0.01)。结论卡巴胆碱能使烧伤大鼠肠系膜细静脉舒张,加快肠系膜微血管血流速度,增加肠道的血液灌注量,具有改善肠道微循环的功能。

Objective To investigate the effects of carbamylchocline on mesenteric microcirculation in burn-injured rats. Methods Adult Wistar rats were randomized to undergo a 30% TBSA Ⅲ thermal injury （Burn） or manipulation without burn（Sham）. The animals were administrated with either carbamylchocline（30 μg/100 g body） or NS 20 min after Burn or Sham. 4 hours later, mesenteric microcirculation was observed by an intravital microscope equipped with a TV camera. The blood flow in intestinal wall was measured with PIMII laster Doppler perfusion Imager. Results （1） In burn-injured group, mean carotid pressure and the internal diameters of venules reduced by 19.3% （ P 〈 0. 01, compared with sham rats） and 9.97% （ P 〈 0.05, compared with sham rats） respectively. After treatment of burn-injured rats with carbamylchocline, mean carotid pressure and the internal diameters of venules increased by 17.88（ P 〈 0.05, compared with burn-injured rats） and 29.42% （P 〈 0.001, compared with burn-injured rats）. （2） The velocity of mesenteric microvessles and the blood flow of intestinal wall in burn-injured rats decreased by 68.08% and 70.66% （ P 〈0.001, compared with sham rats）. In contrast, treatment of burn-injured rats with carbamylchocline prevented the decrease of the velocity and the blood flow（P 〈0. 001, compared with burn-injured rats）. （3） There was a decrease of the amount of capillary network in burn-injured rats（P 〈 0. 001, compared with burn-injured rast）. However, the amount of capillary network in carbamylchocline-treated rats was 1.95-fold more than that in burn-injured rats （P 〈 0.01）. Conclusion Carbamylchocline improves the dysfunction of intestinal microcireulation.