龙牙百合茎尖脱毒快繁及种球培育技术

Studies on in Vitro Virus-free Propagation by Soot-tip and Bulb Culture of Longya Lilium

当龙牙百合鳞茎在58℃下处理10-20min，再在40℃下处理72h；茎尖大小为0．2～0.4mm时的诱导成活率达60％～66．7％。继代增殖培养基为MS＋1．0mg／LBA＋0．1mg／LNAA时，其增殖系数是3．36。培养基中4．5％～5．5％的食用糖和60d的培养时间利于鳞茎数量和质量的增加。移栽后的小鳞茎培育8个月可达鲜重22g／个以上的脱毒种球。

Longya lilium bullets were first treated at 58 %： for 10 - 20 minutes then at 38 ℃ for 72 h. The survival ratio with shoot - tip of 0.2 - 0.4 mm was 60.0%- 66.7%. The reprogactiving coefficient of bulb was 3.36 when a medium for continual culture was MS ＋ 1.0 mg/L BA ＋0.1 mg/L NAA. The quantity and quality of bullet would be increased with a media added with 4.5% - 5.5% sugar and cultured for 60 days. Each transplanted bulb could become virus - free bullet which weighed more than 22 g after 8 months＇ cultivation.