蟾酥注射液对H22腹水型肝癌的抑制作用及对荷瘤小鼠生存周期的影响

Influence of Secretio Bufonis Injection on Mean Survival Time and Carcinostatic Activities in Mice Bearing Intraperitoneal H22 Tumor

目的探讨蟾酥注射液对H22腹水型肝癌小鼠肿瘤抑制作用的作用机理。方法将60只昆明种小鼠随机分为3组。蟾酥注射液组(治疗1组):每次注射蟾酥注射液1.5mL/只;环磷酰胺组(治疗2组):注射环磷酰胺0.5mg/只(用生理盐水组稀释);生理盐水组(对照组):注射生理盐水0.5mL/只。每组20只。各组均隔5d注射1次,共给药3次。每组中取10只最后1次注射后5d,采集血液,检测肿瘤坏死因子;另10只记录小鼠存活情况。结果蟾酥注射液能不同程度延长腹水型肝癌小鼠的生存期,能使肿瘤坏死因子水平明显下降,与对照组比较,P<0.01,治疗组之间比较,P<0.05。结论蟾酥注射液对H22腹水型肝癌小鼠肿瘤有抑制作用,能够延长生存期。

Objective To investigate the mechanism of Secretio bufonis injection on the carcinostatic activities in mice bearing intraperitoneal H22 tumor. Methods Secretio bufonis injection （1.5 mL） was injected into the abdomind cavity of mice once 5 d for 3 times. Cyclophosphamide （0.5 mg/0.5 mL） was injected into other groups. NS （0.5 mL） was injected into control groups. Each group were 20 mice. TNF and survival time of mice were observed. Results Mean survival times of mice bearing intraperitoneal H22 tumor were （28.2±5.8）d for Secretio bufonis group, （27.9±9.7）d for Cyclophospharnide group, （20.7±4.2）d for control group. The content of TNF in mice bearing intraperitoneal H22 tumor were 2.53±0.14 for Secretio bufonis group, 3.02±0.14 for Cyclophospharnide group, 3.32±0.2 for control group. The survival times of mice bearing intraperitoneal H22 tumor were prolonged in the groups of Secretio Cyclophosphamide （P〈0.05）. The content of TNF were significantly inhibited bufonis （P〈0.01）. Conclusion Secretio bufonis injection can inhibit the tumor time of mice bearing intraperitoneal H22 tumor. bufonis （P〈0.01） and in the groups of Secretio and prolong the survival