摘要
比较了各种溶剂系统对高速逆流色谱法分离硫代葡萄糖苷的影响,考察了高速逆流色谱的流速、转速和进样量对提取效果的影响。用V(正丁醇):V(乙腈):V(15%的硫酸铵溶液)= 1:0.5:2.4作为溶剂系统上相作流动相,在体积流量为1.0 mL/min、转速为600 r/min的条件下,成功地从甲醇粗提物中分离了硫代葡萄糖苷,并用高效液相色谱法测定硫代葡萄糖苷,纯度达到95.2%。
In the manuscript, the effects on different solvent system, flow rate, rotary speed and sample value for extract glucosinolate from Eruca satire seed were studied. Glucoerucin was obtained by HSCCC with a binary phase solvent system composed of EtOH-ACN-15% （NH4）2 SO4 （1 ： 0.5 ： 2.4）. The mobile phase was the upper phase and performed at a flow rate at 1.0 mL/min, while the apparatus rotated at 600 r/min. The result of high performance liquid chromatographic revealed that the purity was 95.2%.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第6期13-16,共4页
Journal of Food Science and Biotechnology
基金
浙江省重大科技项目(2004C12005)
