摘要
采用组织破碎,二度硫酸铵盐析,DEAE Sepharose F.F.离子交换层析及Sephadex G—75 Fine凝胶过滤等纯化方法,从猪心中提取得到了苹果酸脱氢酶。提取纯化的苹果酸脱氢酶经SDS—PAGE测定显示为单一条带,相对分子质量为34000。酶活回收率为57.99%,最适pH为8.0,最适温度为50℃。
By tissue crushing, ammonium sulfate duel-precipitation and purification through DEAE Sepharose F. F. Sephadex G-75 filtration, the malate dehydrogenase （MDH） from swine heart was prepared. The purified MDH was illustrated as a single band in SDS--PAGE. molecular weight of MDH subunit was 34 000. The recovery of MDH activity was 57.99%. optimum pH for MDH was 8.0, and the optimum temperature for MDH was 50℃. The The
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第6期90-94,共5页
Journal of Food Science and Biotechnology
关键词
猪心
苹果酸脱氢酶
柱层析
酶学性质
Swine heart
malate
chromatography
character of enzyme
