摘要
运用生物信息学方法,通过构建木聚糖酶进化树,研究了两个家族的分子进化,发现它们进化模式有差别,F/10家族木聚糖酶在进化时间上较早,功能分化不太完全,具有部分糖苷酶属性,在分解产物中含有较多单糖;而G/11家族木聚糖酶在进化时间则相对较近,多在真菌中出现,功能分化较为完全,很少含有糖苷酶的属性,分解产物中低聚寡糖成分较多。F/10木聚糖酶分子进化显示出生物进化来源于高温环境,热稳定性比G/11木聚糖酶高,这与它们三维结构的不同有关。所以,木聚糖酶的进化方向是:功能的进一步分化和底物特异性的进一步增加,这个结果对木聚糖酶的合理应用具有指导意义。
A bioinformatics method was used to study the molecular evolution of xylanase. The evolution model was found to be different between F/10 and G/11 xylanase, this attributes to their different three-dimension structures. The evolutionary trend of xylanase function was hypothesized to be more divergent and more specific from promiscuity. GIll xylanase was found to be a kind of more recent enzyme compared with F/10 xylanase, and the evolutionary route in F/10 xylanase indicated clearly that organism being evolved from eubacteria to fungi, and that xylanase came from a high temperature living organism.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第6期110-116,共7页
Journal of Food Science and Biotechnology
基金
河南省科技攻关项目(072102220001)
河南农业大学博士基金项目(BS200503)
关键词
生物信息学
木聚糖酶
结构
分子进化
bioinformatics
xylanase
structure
molecular evolution
