围生期双酚A暴露对子代大鼠脾脏免疫功能的影响

Effects of Perinatal and Lactational Exposure to Bisphenol A on the Immune Function of Spleen in the Offspring of Rats

为了研究围生期双酚A(BPA)暴露对子代脾脏免疫功能的影响,选择F344大鼠,按体重随机分为对照、低、中、高4个剂量组(BPA暴露剂量分别为0、4、40、400mg·kg-1·d-1),从雌鼠妊娠第0天开始至子鼠出生后30天,每天灌胃染毒1次,观察子鼠脾脏组织形态学的变化,并采用实时定量PCR检测IL-2、IL-12、IFN-γ、TNF-α4种细胞因子mRNA表达的改变.结果表明,1)与对照组相比,低剂量组子鼠脾重、脾脏指数均显著增加(p<0.05),体重无显著变化(p>0.05);中剂量组子鼠脾脏指数显著增加(p<0.05),体重和脾重无显著变化(p>0.05);高剂量组子鼠体重、脾重均显著降低(p<0.05),脾脏指数无显著变化(p>0.05).2)与对照组相比,各暴露组子鼠脾脏IL-2、IL-12、IFN-γ、TNF-α4种细胞因子mRNA表达均显著降低(p<0.01).以上结果提示,BPA可能能够透过胎盘屏障并通过乳汁传递给子代,降低子代的机体免疫功能.

To study the effects of perinatal and lactational exposure to bisphenol A （BPA）on the immune function of spleen in the offspring of rats, Fisher 344 rats were divided into control group, low-dose group, middle-dose group and high-dose group, and were gavaged with 0.4.40.400mg·kg^-1 bisphenol A per day, respectively, from pregnancy to 30 days after the offspring rats were born. After exposure, the changes of histomorphology of spleen in the offspring rats were observed and the mRNA expressions of IL-2,IL-12,IFN-γ,TNF-α of spleen in the offspring rats were detected by real-time RT-PCR. Results showed that, compared with the control group, the spleen index in low-and middle-dose group decreased significantly（p〈0.05 ）. The weight and spleen weight of offspring rats in high-dose group decreased significantly （p〈0.05）. The mRNA expressions of IL-2、IL-12、IFN-γ、TNF-α in all exposure groups decreased significantly （p〈 0.01 ）. These results suggested that BPA could permeate placental barrier and be transferred from exposed animals to their offspring by lactation.