纳米SiO_2与常规SiO_2颗粒对Hela细胞的细胞毒性作用

Cytotoxicity Effects of Nano-SiO_2 and Normal SiO_2 Particles on Hela Cells

为了探讨纳米SiO2和常规SiO2颗粒对Hela细胞的细胞毒性作用,采用不同浓度的纳米SiO2和常规SiO2颗粒(0.05、0.1、0.2、0.4、0.8、1.6μg·μL-1)对Hela细胞进行12h染毒,应用MTT法检测细胞毒性效应.研究发现,较低浓度(≤0.2μg·μL-1)的纳米SiO2和常规SiO2对Hela细胞无明显细胞毒性(p>0.05);较高浓度时,纳米SiO2(≥0.4μg·μL-1)和常规SiO2(≥0.8μg·μL-1)对Hela细胞具有明显细胞毒性作用(p<0.01),并且随浓度增大细胞毒性增强;当浓度≥0.4μg·μL-1时,纳米SiO2的细胞毒性明显高于相同浓度的常规SiO2(p<0.05).以上结果表明,纳米SiO2和常规SiO2颗粒均能对Hela细胞产生细胞毒性,且纳米SiO2的细胞毒性强于常规SiO2;低浓度(≤0.2μg·μL-1)的纳米SiO2和常规SiO2具有很好的生物相容性.

In order to explore and compare the cytotoxicity induced by nano-SiO2 and normal SiO2 particles, Hela cells were exposed to these two particles with different concentrations （0.05、0.1、0.2、0.4、0.8、1.6μg·μL^-1） for 12h. The MTT assay was applied to measure the cytotoxicity. Results showed that, low concentration （≤〈0.2μg·μL^-1） nano-SiO2 and normal SiO2 had no significant toxicity to Hela cells（p〉0.05）. While in the high concentration, nano-SiO2 （≥0.4μg·μL^-1） and normal SiO2 （≥0.8μg·μL^-1）had significant toxicity to Hela cells （p〈0.01）, and the cytotoxicity increased with the increase of concentrations. Nano-SiO2 induced greater cytotoxicity than normal SiO2 at the same concentration（p〈 0.05 ） when the concentration was above 0.4μg·μL^-1. It was concluded that both nano-SiO2 and normal SiO2 particles could induce cytotoxicity to Hela cells, and nano-SiO2 showed greater cytotoxicity than normal SiO2 particles. Low concentration（ ≤ 0.2μg·μL^-1） nano-SiO2 and normal SiO2 were well biocompatible with Hela cells, and this characteristic probably had a good application foreground in biomedical research.