摘要
利用正交实验法探讨纤维素不完全降解物诱导鸡腿菇产纤维素酶的特征.实验结果表明:分子结晶化程度及单体结构组成不同的纤维素原料的不完全降解物对菌种产纤维素酶的诱导有明显差异,其中结晶度低的高粱渣水解液96h可使纤维素酶FP活力高出对照组约30%;其次,水解液浓度和培养条件对菌菇产酶也有一定影响.诱导鸡腿菇产纤维素酶的最适条件为0.5%酸解高粱渣,25℃,100 r/m转速,pH5.5.这可以为食用菌栽培生理的调控提供一定的基础数据.
： This text used Orthogonal design test to research the characteristic of cellulase from Coprinus comatus induced by disintegrated cellulose in the liquid cultivation. The conditions include different type of disintegrated cellulose, temperature, pH and shaker rate. The results showed that the type of disintegrated cellulose was the biggest element to induce cellulase. The next was the concentratior/of cellulose, shaker rate, temperature and pH. The Optimization conditions of induced cellulase from Coprinus comiztus＇were 0. 5% the disintegrated cellulose of sorghum, 25℃, 100 r/m and pH5.5.
出处
《上海师范大学学报（自然科学版）》
2007年第6期63-67,共5页
Journal of Shanghai Normal University(Natural Sciences)
基金
上海市教委青年基金项目(03DQ25)
关键词
鸡腿菇
纤维素降解物
诱导作用
纤维素酶
正交实验
Coprinus comatu
disiniegrate cellulose
induce
Cellulase
Orthogonal design test
