摘要
目的通过检测转化生长因子TGF-βⅡ型受体(TβR-Ⅱ)、Smad4与Smad7蛋白在胃癌组织中的表达,研究它们与胃癌发生、发展的关系。方法应用S-P免疫组化检测方法,对10例正常胃黏膜、10例早期胃癌与45例进展期胃癌组织的TβR-Ⅱ、Smad4与Smad7蛋白的表达进行检测。结果TβR-Ⅱ与Smad4蛋白在正常胃黏膜和早期胃癌中的表达明显高于进展期胃癌,且胃癌分化程度越低,阳性表达率越低(P<0.05)。Smad7蛋白在进展期胃癌中的表达明显高于正常胃黏膜和早期胃癌(P<0.05)。TβR-Ⅱ、Smad4及Smad7蛋白的表达均与淋巴结转移相关。结论TβR-Ⅱ、Smad4与Smad7蛋白三者表达的异常可能与胃癌的发生、发展和转移相关,可能成为研究胃癌分化与转移的生物学标记物。
Objective To study the expression and significance of TβR-Ⅱ （Transforming growth factor βⅡ Receptor）, Smad4 and Smad7 protein in gastric carcinoma tissues and investigate their relationships. Methods The expression of TβR-Ⅱ , Smad4 and Smad7 protein in 10 normal gastric, 10 earlier gastric carcinoma and 45 advanced gastric carcinoma tissues were examined by S-P immunohistochemical method. Results The expression of TβR-Ⅱ and Smad4 protein in normal gastric and earier gastric carcinoma were significantly higher than those in advanced gastric carcinoma. The lower the differentiation of gastric carcinoma, the lower the expressions of TβR-Ⅱ and Smad4 protein. There was much positive rate of Smad7 protein in gastric carcinoma than that in normal gastric mucosa. TβR-Ⅱ , Smad4 and Smad7 protein all had significant correlation with the lymph node metastasis. Conclusion Abnormal expressions of TβR-Ⅱ, Smad4 and Smad7 protein will promote the growth and the lymph node metastasis of gastric cancinoma in some degree, so they can be regarded as very important biologic markers in the study of differentiation and metastasis of gastric cancinoma.
出处
《实用肿瘤学杂志》
CAS
2007年第6期513-515,518,共4页
Practical Oncology Journal
