鼻咽癌细菌L型感染与PCNA、p21、p53表达的关系

Relationship between L-form Bacterial Infection and Expression of PCNA, p21 and p53 in Nasopharyngeal Carcinoma

目的：探讨细菌L型可能致癌的机理。方法;用革兰染色和免疫级化染色技术对69例鼻咽癌、20例鼻咽粘膜慢性炎症进行细菌L型检测，同时对L型阳性和阴性组织的PCNA、P21、P53表达进行对比分析。结果;鼻咽癌组的L型检出率为79.7％，与慢性炎症组（75.0％）无显著性差异（P＞0.05），但鼻咽癌组的PCNA、p21、p53表达部显著高于L型阴性组（P＜0.01）。表明L型感染与鼻咽癌的PCNA、p21、p53过度表达存在着相关性及L型感染的组织处于高增殖状态。结合提示L型感染参与了ras原癌基因激活和p53抑癌基因的失活。基因突变可能是L型致癌作用的机制之一。

Purpose: To investigate the pesible carcinogenesis mechanisms of L-form bacteris. Metbod: Sixty-nine cases of nasopharyopal careinoma (NPC) and 20 cases of chronic nasopharyngitis (NPS) aareisted with bacterial L- form were meined by Gram's stawi and immunohistochemisry. The expresaion of PCNA, p21 and p53 in the cases with bacterial L-form infection and without bacterial L-form ndectbo were observed. Results: It showed that the poritive rate of the infection of bacterial L-forms in NPC group and in NPS groop was 79. 7K and 75. 0% respectively (P＞0.05). The expression of PCNA, p21 and p53 was signifhantly higher in NPC group than that in Nes group (Pwt0.005). The expression of PCNA, p21 and p53 were significantly higher in L-form in feCtbo peitire group than that in ncgative grop(P＜0.01 ). The results edicatc that L-form infection was correisted with expression of PCNA, p21 aed p53. The increasing epithelial cell Proliferation was mpuenced by L-form infectbo and aariated with an increafor risk of carcbo. Conclusbo: It suggsts that ras gene aed p53 gene mutation may be related with L- form infedtbo.Bacterial L-form iofecton may be one of the carcinogenesis of nasopharyngeal.