3个高粱品种及其F_1的过氧化物酶同工酶分析

Isozyme analysis of peroxidase in three sorghum varieties and their F_1 hybrids

采用聚丙烯酰胺凝胶电泳(PAGE)方法,对分别以栽培高粱和甜高粱为母本、以拟高粱为父本进行杂交获得的F1代及其双亲的过氧化物酶同工酶进行分析。结果表明:栽培高粱和甜高粱酶谱类型相近,仅个别酶带活性强弱存在差异,所以两者的亲缘关系可能较近,POD不能作为区分鉴别它们的依据;栽培高粱和甜高粱酶谱比拟高粱少酶带POD-1,与拟高粱的亲缘关系可能较远,酶带POD-1可在一定程度上反映出拟高粱与栽培高粱和甜高粱的区别。杂交F1代的酶谱与父本较相近,既表达出互补双亲酶带的"互补带"POD-1,又表达新的"杂合带"POD-4,但未表达出双亲共有酶带POD-6,因此,可为选育杂交新材料提供依据。

Applying polyacrylamide gel eletrophoresis （PAGE） method, the isozyme of peroxidase （POD） of three sorghum varieties and their F1 hybrids were analyzed, which Sorghum bicolor and Sorghum dochna were used as female parent and Sorghum propinquum as male parent. The results indicated that zymogram type of Sorghum In＇color and Sorghum dochna were similar, and there only existed the difference of a band activity between them. Therefore, the genetic relationship of Sorghum bicolor and Sorghum dochna maybe close and isozyme of peroxidase could not identify them. While Sorghum bicolor and Sorghum dochna was lack of band POD-1 as compared to Sorghum propinquum , and maybe have a far genetic relationship with Sorghum propinquum. So band POD-1 could distinguish Sorghum propinquum and Sorghum bicolor , Sorghum dochna . The zymogram of F1 was similar to male parent, and had complementary bands POD-1 for parents and new heterodimeric band POD-4, while did not express POD-6 which was common for parents. Therefore, the research results could provide reference to breed new hybrid materials.