海人酸致大鼠海马及脑脊液谷氨酸及天冬氨酸变化的意义

Changes of Aspartate and Glutamate in Hippocampus Formation and Cerebrospinal Fluid in Kainite Acid Induced Epilepsy Rat

目的探讨海人酸(KA)致大鼠模型海马及脑脊液谷氨酸(Glu)和天冬氨酸(Asp)变化的意义。方法SD大鼠40只随机分成KA组(KA2μg/kg,海马杏仁核注射后分为6h、1、3、7d组,每组8只)和9g/L盐水对照组(n=8,海马杏仁核注射与致剂等容量的9g/L盐水)。采用高效液相色谱(HPLC)法测定大鼠海马组织及脑脊液兴奋性氨基酸Glu和Asp水平,并进行比较。结果大鼠海马中Glu、Asp水平在观察的24h内随时间推移呈逐步增长趋势,但与对照组相比无显著性差异,3d后仅Glu较对照组明显上升。其脑脊液Glu、Asp水平在致后6h与对照组比较有显著差异,且7d内保持上升趋势。结论癫大鼠脑脊液兴奋性氨基酸水平变化较海马组织早,其中Glu增高尤为突出。

Objective To explore the changes of contents of glutamate （Glu） and aspartate （Asp） in hippocampus formation and cerebrospinal fluid （CSF） in kainite acid（KA） induced epilepsy rats. Methods SD rats （n = 40） were divided into 2 groups randomly：the KA group [ intracerebroventricular injection （icy） of KA, 2 μg/kg ] and control group（ icy of NS）. KA group were divided into 4 groups at 6 h, 1, 3 and 7 d, each group 8 rats. High pressure liquid chromatgraphy （ HPLC ） was used to assess the concentrations of Glu and Asp in hippocampus formation and CSF. Results In the hippocampus, the contents of Glu and Asp increased continuously I d after seizure , but not different from those of control group. Three days later, only Glu became significantly different from control group. However, the contents of Glu and Asp in the CSF were significantly different from the control 6 h after seizure. Conclusion The contents of excite amino acid （ especially Glu） in CSF increase immediately after KA injection, which are earlier than those in hippocampus formation.