杜仲翅果蛋白质提取研究

Extraction of Eucommia Key-fruit Protein

以杜仲翅果籽粕为原料，采用单因素试验和正交试验，对碱液法提取杜仲翅果蛋白的工艺条件进行了探讨。测得杜仲翅果蛋白质等电点为pH3．8，加入0．3％Na2SO3可有效抑制提取液的褐变。碱液法浸提杜仲翅果蛋白质的优化工艺条件为：浸提液pH为9，料液比为1：20，浸提温度45℃。浸提时间90min。浸提3次。在此条件下，杜仲翅果蛋白提取率可达89．17％，所得产品的蛋白质含量达94．5％。

Using Eucommia Key-fruit seed dregs as material, the technical conditions of lye extracting protein were investigated by single factor and orthogonal experiments. The result showed that the protein isoelectric point is pH3.8, and 0.3% Na2SO3 could restrain effectively the browning of extraction liquid. The optimal extraction technology conditions as follow： pH of the extraction liquid is 9, the ratio of material to liquid is 1 ： 20, the extracting time is 90 min, the extraction temperature is 45 ℃ and the extraction degree is three times. In these conditions, the protein extracting yield is 89.17%, and the protein content of the product is 94.5%.