Ⅲ期鼻咽癌中p53与LMP-1蛋白表达与预后的关系

Relationship between the expression of protein p53 or LMP-1 and the prognosis of patients with stage Ⅲ nasopharyngeal carcinoma

目的探讨Ⅲ期鼻咽分化型非角化性鳞癌(DNKSC)中p53蛋白与LMP-1蛋白表达与生物学行为及预后的关系。方法应用免疫组织化学EnVision法检测58例Ⅲ期鼻咽DNKSC标本。结果58例Ⅲ期鼻咽DNKSC中,p53蛋白表达总阳性率为65.5%(38/58);生存期在5年以下患者阳性率为82.1%(32/39)与5年以上患者阳性率31.6%(6/19)比较差异有显著性意义(P<0.05);有颅底侵犯组p53蛋白表达阳性为74.4%(29/39)与无颅底侵犯组47.4%(9/19)比较差异有显著性意义(P<0.05)。LMP-1蛋白总阳性率为72.4%(42/58),与p53蛋白表达呈正相关关系(r=0.504)。结论Ⅲ期鼻咽DNKSC中p53蛋白表达与LMP-1蛋白表达相关;有颅底侵袭组p53蛋白表达阳性率高于无颅底侵袭组;p53蛋白表达阳性随着患者生存期的延长而降低。

Objective To investigate p53 protein and LMP-1 protein expression in stage m nasopharyngeal differentiated non-keratinizing squamous carcinoma （DNKSC） and their correlation with biological behavior and prognosis. Methods Immunohistochemistry （Envision） assay was applied to detect p53 protein and LMP-1 protein expression in 58 cases. Results The p53 protein expression showed general positive rates of 65.5 % （38/58 ） in patients with nasopharyngeal DNKSC, being signifi- cantly higher in patients surviving less than 5 years （32/39, 82.1% ） than those surviving longer than 5 years （6/19, 31.6% , P 〈 0. 05）. It was closely correlated with the local invasion to the cranial base. There was a significant difference between inva- sion to the cranial base （29/39, 74.4 % ） and non-invasion （9/19, 47.4% , P 〈 0. 05）. The expression of LMP-1 protein accounted for 72.4 % （ 42/58 ） , exhibiting a positive correlation with the expression of p53 protein （ r=0. 504）. Conclusion In stage m nasopharyngeal DNKSC, the expression of p53 protein is related to the expression of LMP-1 protein. The expression of p53 protein declines as the prolongation of survival, and the p53 protein expression is higher in the local invasion group.