二硫苏糖醇对大鼠硒性白内障的抑制作用

Inhibitory action of dithiothreitol on selenite-induced cataract in rats

目的:探讨自行配制的二硫苏糖醇(DTT)滴眼液对SD大鼠硒性白内障(模拟老年性白内障)的抑制作用。方法:对出生后12d的SD乳鼠(约25g)行皮下注射Na_2SeO_3(0.2ml/次,隔天注射1次,共计3次,累计剂量约210μg),注射开始后6d,将发生硒性白内障大鼠左眼设为对照组,右眼作药物干预组,滴眼4次/d(分别滴入生理盐水及25mmol/L的DTT),每次1滴,持续用药3周;定时对大鼠双眼散瞳作裂隙灯检查,以游标尺测量大鼠眼晶状体核性斑块最大直径变化。另取硒性白内障乳鼠分别设为DTT(n=89)、吡诺克辛(PDE,n=22)及吡诺克辛钠(PSED,n=24)干预组及生理盐水对照组(n=45),分别使用25mmol/LDTT、日本产吡诺克辛滴眼液、国产吡诺克辛钠滴眼液。滴眼4次/d(同组乳鼠左、右眼滴相同的药物或生理盐水),每次每只眼1滴(约40μl),持续用药3周。定时散瞳后,用裂隙灯检查大鼠双眼皮质病变、混浊程度。结果:(1)DTT滴眼液可以明显抑制白内障大鼠晶状体核性混浊斑块的发展;(2)DTT滴眼液可以有效降低白内障大鼠晶状体伴发皮质混浊的发生率;(3)DTT滴眼液抗硒性白内障晶状体混浊的效果优于其他临床常用的抗老年性白内障药物PDE和PSED。结论:DTT滴眼液对SD大鼠硒性白内障具有良好的抑制作用。

Objective：To study the inhibitory action of self-designed dithiothreitol（DTT） eye drops on selenite-induced rat cataract（simulating the senile cataract）. Methods： Twelve-day-old SD rats （body weight about 25 g） were injected with Na2 SeO3 subcutaneouly, quaque die aherna, for 3 times （0.2 ml/time, with a total amount of 210 μg）. Six days later over 90% of rats developed nuclear cataracts. The right eyes of the rats were treated with 25 mmol/L DTT eye drops （4 times/day, l drop/time） for 3 weeks. The left eyes were taken as control and were treated with normal saline at the same dosage. The lens were examined using slit lamp and the diameter of the cataract plaques were measured using vernier cursor. The eyes of cataract models were also treated with 25 mmol/L DTT（n = 89）, pirenoxine eye drops （PDE, Japan, n = 22）, pirenoxine sodium eye drops （PSED, China, n=24, 2 died during the experiment）, and normal saline （n： 45）. Each eye was administered with 1 drop/time, 4 times/day for 3 weeks. Then slit lamp was used to examine the cortical lesions of both eye and the lens opacity. Results： The DTT eye drops significantly reduced the diameter of the cataract plaques and significantly reduced the rate of the cortical opacity after the nuclear cataract. The inhibitory action of DTT eye drops on the selenite-induced cataract was superior to pirenoxine eye drops（Japan） and pirenoxine sodium eye drops（China）. Conclusion： Our study shows that the DTT eye drops has satisfactory inhibitory action on selenite-induced cataract in rats.