海藻酸盐在软骨组织工程中的应用

Application of alginate in cartilage tissue engineering

学术背景:以生物材料作为细胞生长的三维支架,利用组织工程技术生产用于移植治疗的软骨组织是当前研究的重点。目的:对海藻酸盐在软骨组织工程中的应用情况作简述。检索策略:应用计算机检索PUBMED数据库1966-10/2007-07的相关文章,检索词为"Tissue engineering,Cartilage tissue engineering,Alginate",并限定文章语言种类为English。同时计算机检索万方及CNKI数据库1979-10/2005-10的相关文章,检索词为"组织工程、软骨、海藻酸盐",并限定文章语言种类为中文。对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与海藻酸盐在软骨组织工程中的应用情况相关。排除标准:重复研究或不相干文章。共收集到314篇相关文献,258篇文献符合纳入标准,排除的56篇文献为内容陈旧或重复。符合纳入标准的文献中,精选其中55篇用作研究分析。文献评价:文献的来源主要是海藻酸盐在软骨组织工程中的应用情况的研究。55篇符合纳入标准的文献中,中文23篇,英文32篇,其中动物实验和在体、离体、细胞学实验50篇,综述、述评类文献5篇。资料综合:通过对藻酸盐的生物学特性、不同组织来源的种子细胞与藻酸盐载体、不同塑形形式的藻酸盐载体及相关生物刺激因子(素)在以藻酸盐为载体的软骨组织工程中的应用等方面作了综述分析。结论:藻酸盐载体来源丰富,具有易塑形、易于细胞吸附、营养物质易于渗透等特点;但藻酸钙也存在体内吸收差和有一定抗原性等缺点。海藻酸盐构建的组织工程化软骨在力学性能、组织结构等方面与正常软骨相比还有一定的差距,有待进一步研究提高。

BACKGROUND： Current researches emphasize on the transplantation therapy for cartilage tissue by tissue engineering technique, taking biomaterials as three-dimensional scaffold of cell growth. OBJECTIVE： To summarize the application of alginate in the cartilage tissue engineering. RETRIEVE STRATEGY： A computer-based search of PubMed Database was undertaken to identify the articles published between October 1966 and July 2007, with the key words ＂Tissue engineering, Cartilage tissue engineering, Alginate＂ in English. Meanwhile the related articles were retrieved in Wanfang and CNKI from October 1979 to October 2005, with the key words ＂tissue engineering, cartilage, alginate＂ in Chinese. Data were checked primarily and every literature was looked up for the full texts. Inclusive criteria： the articles related to the application of alginate in cartilage tissue engineering. Exclusive criteria： repeated researches or unrelated articles. Totally 314 related articles were collected, 56 of them were excluded due to the outdated or repetitive contents and 258 papers were suitable for data pooling. Of which, 55 papers were carefully analyzed to the content. LITERATURE EVALUATION： These papers mainly focused on the application of alginate in cartilage tissue engineering. Among the 55 selected articles, including 23 in Chinese and 32 in English, there were 50 ones of animal experiment and in vivo, ex vivo, cytological trials, while other 5 were reviews and commentaries. DATA SYNTHESIS： The biological character of alginate, seeding cells from different tissues in combination with alginate, different molding forms of alginate and related biostimulating factors, as well as their application in cartilage tissue engineering were described briefly. CONCLUSION： Alginate is abundant and easy for modeling, cell sticking and nutrient infiltration. But it is hard for in vivo absorbance and has little antigenicity. In aspects of biomechanical property and tissue histological structure, there is also room for improving alginate-based tissue engineered cartilage to a normal biological cartilage.