期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

磷酸二酯酶5选择性抑制剂zaprinast对小鼠卵母细胞成熟的影响 被引量：3

Effects of Phosphodiesterase Type-5 Inhibitor on Mouse Oocyte Maturation In Vitro
下载PDF
导出
摘要 目的研究磷酸二酯酶5(PDE5)选择性抑制剂扎普司托(zaprinast)对小鼠卵母细胞体外成熟的影响。方法采用小鼠卵母细胞自发成熟、促卵泡刺激素(FSH)诱导次黄嘌呤(HX)阻滞成熟以及卵泡卵母细胞成熟三种体外培养模型。结果10μmol/L的zaprinast不仅能显著抑制小鼠卵丘卵母细胞复合体(COCs)和裸卵(DOs)的自发成熟(P<0.01),而且还能显著抑制FSH对HX阻滞的COCs的以及卵泡卵母细胞成熟的促进效应(P<0.01)。结论PDE5能促进小鼠卵母细胞的体外成熟。 Objective To study the effects of the selective inhibitor of PDE5 on mouse oocyte maturation in vitro. Methods Mouse cumulus-oocyte complexes （COCs） or denuded oocytes （DOs） were collected and cultured in the medium supplemented with the selective inhibitor of PDE5 and the rates of germinal vesical breakdown （GVBD） and first polar body （PB1） extrusion were observed at the end of the culture. In addition, a mouse follicle culture model was also used. Results In isolated oocytes, PDE5 inhibitor zaprinast prevented significantly both spontaneous oocyte meiotic maturation and FSH-induced HX-arrested meiotic maturation. In addition, treated the follicles with the type 5 inhibitor significantly inhibited the follicle-enclosed oocyte maturation induced by FSH. Conclusion Collectively, these results suggest that PDE5 induces mouse oocyte maturation.
作者 卜淑敏 张颢 季刚
机构地区 首都体育学院科研处重点实验室
出处 《中国比较医学杂志》 CAS 2007年第12期702-705,共4页 Chinese Journal of Comparative Medicine
基金 北京市教委科技发展规划资助项目(编号:KM200710029001)
关键词 磷酸二酯酶 cAMP CGMP 卵母细胞 小鼠 PDE cAMP cGMP Oocytes Mice
分类号 Q492.5 [生物学—生理学]
  • 相关文献

参考文献15

  • 1Richard FJ. Regulation of meiotic maturation[ J ]. J Anim Sci, 2007, 85 ( 13 Suppl) : E4 - 6.
  • 2Mahrco C, Carsten B, Andersen, et al. Role of cyclic nucleotide signaling in oocyte maturation[ J ]. Mol Endocfinol, 2002,187 : 153 - 159.
  • 3Tsafriri A, Chun SY, Zhang R, et al. Oocyte maturation involves compartmentalization and opposing changes of cAMP levels in follicular somatic and germ ceils : studies using selective phosphodiesterase inhibitors [ J ]. Dev Biol, 1996,178 : 393 - 402.
  • 4Palmer MJ, Bell AS, Fox DN, et al. Design of second generation phosphodiesterase 5 inhibitors [J]. Curr Top Med Chem. 2007,7 (4) :405 - 419.
  • 5Mayes MA, Sirard MA. Effect of type 3 and type 4 phosphodiesterase inhibitors on the maintenance of bovine oocytes in meiotic an'est[J].Biol Reprod ,2002,66: 180 - 184.
  • 6Thomas RE, Armstrong DT, Gilchrist RB. Differential effects of specific phosphodiesteraase isoenzyme inhibitors on bovine oocyte meiotic maturation [ J ]. Dev Biol, 2002,244 : 215 - 225.
  • 7Bilodeau-Goeseels S, Effects of phosphodiesterase inhibitors on spontaneous nuclear maturation and cAMP concentrations in bovine oocytes[ J ]. Theriogenology ,2003,60:1679 - 1690.
  • 8Masciarelli S, Homer K, Chengyu LC, et al. Cyclic nucleotide phosphodicsterase 3A-deilcient mice as a model of female infertility [J]. J Clin Invest,2004,114:196 - 205.
  • 9Thomas RE, Thompson JG, Armstrong DT, et al. Effect of specific phosphodiesterase isoenzyme inhibitors during in vitro maturation of bovine oocytes on meiotic and developmental capacity [ J ]. Biol Reprod,2004,71 : 1142 - 1149.
  • 10Omori K, Kotera J. Overview of PDEs and their regulation. Circ Res. 2007,100 (3):309 - 327.

同被引文献33

  • 1张春和,李焱风,赵华萌.中药联合安特尔治疗迟发性睾丸功能减退30例疗效观察[J].中国男科学杂志,2007,21(6):46-47. 被引量：8
  • 2Francis S H, Lincoln T M, Corbin J D. Characterization of a novel cGMP binding protein from rat lung[J]. J Biol Chem, 1980, 255(2): 620-626.
  • 3McAllister-Lucas L M, Sonnenburg W K, Kadlecek A, et al. The structure of a bovine lung cGMP-binding, cGMP-speeific phosphodiesterase deduced from a cDNA clone[J]. J Biol Chem, 1993, 268(30): 22863- 22873.
  • 4Rosen R C, Kostis J B. Overview of phosphodiesterase 5 inhibition in erectile dysfunction[J]. Am J Cardiol, 2003, 92(9A): 9M-18M.
  • 5Boolell M, Allen M J, Ballard S A, et ai. Sildenafil: an orally active type 5 cyclic GMP-specific phosphodiesterase inhibitor for the treatment of penile erectile dysfunction[J]. Int J Impot Res, 1996, 8(2): 47-52.
  • 6Rotella D P. Phosphodiesterase 5 inhibitors: current status and potential applications [J]. Nat Rev Drug Discov, 2002, 1(9): 674-682.
  • 7Moor R M, Heslop J P. Cyclic AMP in mammalian follicle cells and oocytes during maturation[J]. J Exp Zool, 1981, 216(1): 205-209.
  • 8Schultz R M, Montgomery R R, Belanoff J R. Regulation of mouse oocyte meiotic maturation: implication of a decrease in oocyte cAMP and protein dephosphorylation in commitment to resume meiosis[J]. Dev Biol, 1983, 97(2): 264-273.
  • 9Bornslaeger E A, Schultz R M. Regulation of mouse oocyte maturation: effect of elevating cumulus cell cAMP on oocyte cAMP levels[J]. Biol Reprod, 1985, 33(3): 698-704.
  • 10Tornell J, Billig H, Hillensjo T. Regulation of ooeyte maturation by changes in ovarian levels of cyclic nucleotides[J]. Hum Reprod, 1991, 6(3): 411-422.

引证文献3

二级引证文献7

中国比较医学杂志
2007年 第12期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部