检测H5N1型高致病性禽流感病毒抗体的间接ELISA方法的初步建立

Establishment of Indirect ELISA to Detect H5N1 Virus Antibody

目的建立一种快速检测H5N1病毒感染的血清学方法。方法以H5N1病毒作为抗原包被96孔板,与待检血清作用后,以辣根过氧化物酶标记山羊抗小鼠IgG作为二抗,用ELISA方阵法确定最佳抗原、血清稀释浓度。结果感染7 d小鼠血清中抗体水平很低,感染14 d血清抗体水平较高,最佳抗原稀释度1∶100,最佳血清稀释度1∶200。结论这种快速检测H5N1病毒的诊断方法直接有效、方便易行。

Objective To develop a rapid method for detecting H5N1 virus infection. Methods H5N1 virus antigen reacted with the antibody in the serum samples, Goat anti-mouse antibody labeled with horseradish peroxidase was used to detact the antibody in samples, The best dilution concentration of the antigen and sera were chosen by ELISA phalanx method. Results The level of antibody in the mouse sera at 14 days after inoculation was higher than that in mice at 7 days after inoculation. The best dilution concentration of antigen and sera was 1 ： 103 and 1 ： 203. Conclusion The method developed in this study can be used to detect H5N1 virus infection efficiently and easily,and may benefit to the early detection of H5N1 infection.