转绿色荧光蛋白基因小鼠子宫内膜异位症模型的建立和评价

Evaluation and Establishment of Endometriosis Model by using Transgenic Mice Expressing GFP

目的建立小鼠子宫内膜异位症种植模型,探讨荧光检测异位子宫内膜的方法。方法将转绿色荧光蛋白(GFP)基因小鼠的子宫内膜剪碎后,注射入C57BL/6小鼠的腹腔,喂养含17-β-雌二醇的无菌水,3周后剖检小鼠,在活体荧光成像系统下观察腹腔的绿色荧光,并做免疫组化染色。结果在移植转GFP基因小鼠子宫内膜的C57/BLC57BL/6小鼠的腹腔内存在绿色荧光,且免疫组化证实该异位内膜组织中表达GFP,而对照组均阴性。结论转GFP基因小鼠子宫内膜模型可清晰地显示异位内膜病灶,具有良好的应用前景。

Objective To develop the endometriosis model in the mice, and to investigate the methods of analyzing the ectopic endometrium using the fluorescent imaging. Methods Endometrial fragment obtained from the transgenetic mice treated by Green Fluorescent Protein （GFP） and injected into the peritoneal cavity of C57BL/6 mice. These recipient mice were raised with water supplemented by E2 for three weeks. Then the mice were euthanized, and we observed the intensity of the GFP of the peritoneal cavity under fluorescence microscopy. Results The green fluorescence does exist in the peritoneal cavity of the mice and we have proved that the ectopic endometrium expressed the GFP by the immunohistochemistry. Conclusion The model developed by using the transgeneic mice expressing GFP would be available for exact evaluation of endometriotic lesions, which is prospective.