小鼠哮喘模型气道上皮内肥大细胞数量增多

Intraepithelial mast cells increase in pulmonary airways of asthma model mouse

目的:观察小鼠哮喘模型气道卜皮内肥大细胞的数量,进一步阐明肥大细胞在鸡卵清蛋白(OVA)诱导的小鼠哮喘模型中的作用。方法:OVA诱导建立小鼠哮喘模型,H-E染色病理切片观察肺部感染情况,甲苯胺蓝染色法观察气道上皮内肥大细胞数量的变化。结果:哮喘模型组小鼠肺部炎症明显,在正常组和对照组小鼠气道上皮内没有发现肥大细胞,但在模型组小鼠气道上皮内肥大细胞的数量随OVA的激发次数增加而增多。结论:在OVA诱导的小鼠哮喘模型气道上皮内肥大细胞随着激发次数的增加而增多。气道上皮内肥大细胞在OVA诱导的哮喘模型起重要作用。

Objective; To investigate the number of mast cells and clarify the possible role of mast cells in the development of ovalbumin （OVA）-induced asthma model in vivo. Methods： A routine model of OVA-induced asthma was established, and the lung inflammation was assayed by hematoxylin--eosin （H-E） staining. The intraepithelial mast cells were stained by toluidine bule in the lungs. Results： The lung inflarrmiation was significantly increased in asthma model group compared with the control group. There were no intraepithelial mast cells in the lungs of the normal and control mice. The number of intraepithelial mast cells increased in proportion to times of OVA challenge in the epithelium of the lungs. Conclusion ： These results suggest that the lungs in the OVA-induced asthma model increased along with the challenge times. So the intraepithelial mast cells may play an important role in the OVA-induced asthma model.