恒河猴经瞳孔温热疗法与热休克蛋白相关研究

Expression of heat shock protein 70 after transpupillary thermotherapy

目的探讨恒河猴经瞳孔温热疗法（transpupillary thermotherapy，TTT）治疗后视网膜和脉络膜组织热休克蛋白70（heat shock protein 70，HSPT0）表达。方法利用恒河猴做动物模型。于1TIrr治疗后不同时间点（1h、1d、1周、2周、1月、4月）摘除双眼固定。应用免疫组化技术，分析TTT治疗对猴眼视网膜和脉络膜组织HSP70的影响。结果TTT治疗后，于1d起，在强反应光斑边缘视网膜和脉络膜有HSP70的表达，4个月时仍有弱表达。弱反应光斑1d时，在视网膜全层及脉络膜均有HSP70表达，4个月时消失。同时,TTT治疗可以引起视网膜不同程度组织病理学的损害。结论TTT可以引起视网膜、脉络膜组织病理学损害。局部温度的升高会诱导视网膜、脉络膜内源性HSP70的产生，并且这种表达在激光治疗后1～4个月的猴眼中仍可以存在。

Objective To discuss the expression of heat shock protein 70 （HSP70） at chorioretinal layers after transpupillary thermotherapy （TIT）. Methods Henghe-Monkey was anesthetized and TIT was performed on both eyes.At different time courses（ 1 hour, 1 day, 1 week,2 weeks,4 weeks, and 4 months） after laser irradiation, a histological study was performed and immunohistochemistry was done to analysis the expression of HSP70 on retina and choroid. Results From 1 day to 4 weeks after TIT treatment, there were strong HSPT0 expression on both retinal and choroidal layers,including capillary endothelial cells. At 4 months, there was still faint staining of HSP70 on retinal layer. On faint spot of laser burn, the immunostaining of HSP70 was seen on both retinal layer and choroidal cells. At 4 months, no HSP70 immunoreactivity was detected. Conclusion TIT can cause histological damage on retina and choroid, and can induce overexpression of HSP70, which can maintain for 1 month to 4 months after transpupillary thermotherapy.