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蛋白质组学在眼疾病中的应用进展

Proteomics advancements in ocular diseases
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摘要 蛋白质组学是研究细胞、组织和器官内所有蛋白质的组成及其动态变化的科学,是在蛋白质水平上定量的、动态的、整体的研究生物体,可以从更深层次上获得对细胞生理和生物化学过程、调控网络以及疾病过程的广泛而完整的认识。蛋白质组学在眼科尚处于起步阶段,现就蛋白质组学在眼科疾病中的应用进展作一综述。 Proteornics is a science about the composition of cell, organization and organ and their dynamic changes. It can study the organism quantitatively, dynamically and integrated so that to know the cell physiology and biochemistry, regulation net and diseases generally and completely. However, it is at primary stage in ophthalmology. Now, this article reviews the proteomics advancements in ocular diseases.
作者 于靖 王方
出处 《眼科新进展》 CAS 2007年第12期945-948,共4页 Recent Advances in Ophthalmology
基金 上海市教委重点实验室基金资助(编号:2006BZ068)~~
关键词 蛋白质组学 眼科学 蛋白质分析 proteomics ophthalmology protein analysis
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参考文献32

  • 1Fields S. Proteomics in genomeland[ J]. Science 2001 ;291 (5507) : 1221-1224.
  • 2Stults JT,Arnott D. Proteomics[ J]. Methods Enzymol 2005 ; 402:245-289.
  • 3Arthur JM. Proteomics[J]. Curt Opin Nephrol Hypertens 2003;12(4) :423-430.
  • 4Gygi SP,Rist B,Grlflln TJ, Eng J,Aebersold R. Proteome analysis of low-abundance proteins using multidimensional chromatography and isotope-coded atPmity tags[ J]. J Proteome Res 2002 ; 1 ( 1 ) :47-54.
  • 5Keough T, Youngquist RS, Lacey MP. A method for highsensitivity peptide sequencing using postsource decay ma- trix-assisted laser desorption ionization mass spectrometry[J]. Proc Natl Acad Sci USA 1999 ;96( 13 ) :7131-7136.
  • 6Lee WC, Lee KH. Applications of aiPmity chromatography in proteomics [ J]. Anal Biochem 2004 ;324 ( 1 ) : 1-10.
  • 7Issaq HJ,Chem KC,Janinl GM,Conrads TP,Veenstra TD. Multidimensional separation of peptides for effective proteomic analysis[J]. J Chromatogr B Analyt Technol Biomed Life Sci 2005 ;817( 1 ) :3547.
  • 8Grus FH, Podust VN, Bruns K, Lackner K, Fu S, Dalmasso EA, et al. SELDI-TOF-MS ProteinChip array profiling of tears from patients with dry eye [ J]. Invest Ophthalmol Vis Sci 2005 ;46(3 ) :863-876.
  • 9Shimmura S, Miyashita H, Higa K, Yoshida S, Shimazaki J, Tsubota IL Proteomic analysis of soluble factors secreted by limbal fibroblasts[ J]. Mol Vis 2006 ; 12:478-484.
  • 10Thompson LJ, Wang F, Proia AD, Peters KG, Jarrold B, Greis KD, et cd. Proteome analysis of the rat cornea during angiogcnesis [ J]. Proteomics 2003 ;3 ( 11 ) :2258 -2266.

二级参考文献16

  • 1姚军平,阴正勤,王仕军.蛋白质组学技术分析眼优势柱可塑性相关蛋白△[J].眼科新进展,2006,26(3):167-170. 被引量:1
  • 2Wasinger VC,Cordwell SJ,Cerpa-Poljak A,Yan JX,Gooley AA,Wilkins MR,et al.Progress with gene-product mapping of the Mollicutes:Mycoplasma Genitalium[J].Electrophoresis 1995;16(7):1090-1094.
  • 3Poulaki V.Microdissection,microarrays and proteomics:a new approach to the study of eye diseases[J].Graefe Arch Clin Exp Ophthalmol 2003;241(7):527-528.
  • 4Ouchi M,West K,Crabb JW,Kinoshita S,Kamei M.Proteomic analysis of vitreous from diabetic macular edema[J].Exp Eye Res 2005;81(2):176-182.
  • 5Neuhoff V,Arold N,Taube D,Ehrhardt W.Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250[J].Electrophoresis 1988;9(6):255-262.
  • 6Tom B,Tirra S.2-D electrophoresis using immobilized pH gradients,principles and methods[M].Amersham Pharmacia Biotech Inc;1998:36.
  • 7MacCoss MJ,McDonald WH,Saraf A,Sadygov R,Clark JM,Tasto JJ.Shotgun identification of protein modifications from protein complexes and lens tissue[ J ].Proc Natl Acad Sci USA 2002 ;99 (12):7900-7905.
  • 8Kamei A,Takamura S,Nagai M,Takeuchi N.Phosphoproteome analysis of hereditary cataractous rat lens alphacrystallin[ J].Biol Pharm Bull 2004 ;27 (12):1923-1931.
  • 9Lampi KJ,Shih M,Ueda Y,Shearer TR,David LL.Lens proteomics:analysis of rat crystallin sequences and two-dimensional electrophoresis map[ J].Invest Ophthalmol Vis Sci 2002 ;43 (1):216-224.
  • 10Ueda Y,Duncan MK,David LL.Lens proteomics:the accumulation of crystallin modifications in the mouse lens with age[ J].Invest Ophthalmol Vis Sci 2002 ;43 (1):205-215.

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