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猪源H9N2亚型流感病毒HA基因的序列测定及真核表达 被引量：1

Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine
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摘要 利用RT-PCR扩增猪流感病毒A/Swine/Zhejiang/7/2004(H9N2)(SW/ZhJ7/04)HA基因,测序后将其克隆到真核表达载体pCAGGS上,经酶切分析、PCR鉴定和测序验证,得到重组表达质粒pCAGGS-HA,之后将其转染293T细胞,48h后收集转染细胞样品,进行SDS-PAGE分析,结果显示HA蛋白获得了表达。经Western blot检测,结果表明pCAGGS-HA能够在体外瞬时表达具有免疫学活性的HA蛋白,通过免疫小鼠,对其免疫效果进行了初步研究。 HA gene of SW/ZhJ7/04 was amplified by RT-PCR, sequenced, and cloned into recombinant plasmid pCAGGS-HA. The 293T cells monolayer were transfected with plasmid pCAGGS-HA, expression of the HA protein was detected by SDS-PAGE 48 h after transfection and its immunogenic activity was confirmed by Western blot assay. The immunogenicity of the plasmid pCAGGS-HA was evaluated in mice.
作者 韩庆功 乔传玲 杨焕良 陈艳 辛晓光 丁选亚 陈化兰
机构地区 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业部动物流感重点开放实验室
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2007年第12期916-919,共4页 Chinese Journal of Preventive Veterinary Medicine
基金 国家科技攻关计划项目(2004BA519A55)
关键词 猪流感病毒 HA基因 序列测定 真核表达 swine influenza virus HA gene sequencing eukaryotic expression
分类号 S852.659.5 [农业科学—基础兽医学]
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参考文献13

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共引文献381

同被引文献14

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二级引证文献10

中国预防兽医学报
2007年 第12期

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