我国天津地区莱姆病螺旋体宿主动物的初步调查

Preliminary Investigation on Reservoir Hosts of Borrelia Burgdorferi in the Area of Tianjin

目的了解天津地区莱姆病螺旋体的宿主动物的感染情况。方法采用间接免疫荧光抗体法(IFA)对野鼠、野兔、牛、羊、狗进行了莱姆病螺旋体感染的血清学检测;用病原分离培养方法和PCR方法对野鼠进行了病原学检测。并通过基因测序方法确定基因型。结果野鼠的血清莱姆病感染阳性率最高为32.00%(32/100);并从大林姬鼠、社鼠、小林姬鼠的脾脏和/或肾脏中检查到莱姆病螺旋体的特异片断。通过序列同源性分析确定为Borreliagarinii基因型。结论首次从病原学证实天津林区鼠类为莱姆病螺旋体的重要储存宿主。

Objective To understand the status of the reservoir hosts of Borrelia hurgdorfefi in Tianjin. Methods Indirect immunofluorescence assay was used to test serums of the reservoir hosts including mrs. rabbits, cows, sheep and dogs so that we can know their infectious rates of Borrelia burgdorferi. The methods of isolation and PCR were used to test Borrelia burgdofferi in rats. The sequence analysis was used to identify the genotype. Results The infectious rate of rats from the forest areas was 32.50%. There were five positive samples respectively from Apodemus peninsulae, Rattus nivivente, and Apodemus sylvaticus.The sequence analysis shows that they all belong to Borrelia garinii. Conclusion It was the first time to report that rats might serve as primary reservoir hosts for Borreila burgdofferi in Tianjin.